| Project/Area Number |
24659919
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
MAEDA Nobuko 鶴見大学, 歯学部, 教授 (10148067)
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| Co-Investigator(Kenkyū-buntansha) |
OHSHIMA Tomoko 鶴見大学, 歯学部, 准教授 (50233101)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ストレス / 小児 / う蝕 / 唾液 / 精神的ストレス / 小児ストレス応答 / 口腔環境 |
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| Research Abstract |
For the purpose to establish a diagnostic method for a child's psychological stress noninvasively, a survey of the salivary microRNA as a biomarker was carried out.
The subjects were the elementary school juvenile first-fifth graders. In the subjects, 30 children who had and counseled problem were classified into the stress group, and 30 stable children were into the control group.
Although the dental caries experience number of teeth was twice higher in the stress group than the control group, there was no difference in the number of cariogenic pathogens, the amount of salivary secretion and saliva buffer capacity. Then, the saliva microRNA extraction / purification methods were established and the expression profile was compared using an array. The expression patterns differed in ten sorts of microRNAs. The result suggested that microRNAs are useful as a cluster marker for diagnosis of children's psychological stress.
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