| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Research Abstract |
Multiple lines of recent evidences strongly suggested that nitric monoxide, carbon monoxide, and hydrogen sulfide (H2S) have come to known as the gasotransmitters worked for various cellular functions. Persistent extension stress induce the mRNA expression of cystathionine-b-synthase (CBS) and Nox2 by 3 times and 1.8 times, respectively, after 4 hours in mouse vascular endothelial cell line, UV-2 cell cultured on stress chamber. On the contrary, the extension stress did not induced mRAN expression of eNOS. We examined the effect of the extension stress on the mRNA expression of various redox-related genes in human condroblastic cell line, ATDC5 cell. The extension stress also induced Nox2, iNOS, eNOS, and CBS mRNA expression after 2 to 4 hours, but not cystathionine-v-lyase. These evidences suggested that persistent extension stress induced production of endogenous H2S.
|
