The role of novel gasotransmitter hydrogen sulfide on inflammatory response in oral area

• Project/Area Number: 24659937
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Social dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: AKIFUSA Sumio 九州歯科大学, 歯学部, 教授 (40295861)
• Co-Investigator(Kenkyū-buntansha): NISHIHARA Tatsuji 九州歯科大学, 歯学部, 教授 (80192251)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 硫化水素 / gasotransmitter / 歯周炎 / 顎関節炎 / 歯周疾患 / 関節炎

Research Abstract

Multiple lines of recent evidences strongly suggested that nitric monoxide, carbon monoxide, and hydrogen sulfide (H2S) have come to known as the gasotransmitters worked for various cellular functions. Persistent extension stress induce the mRNA expression of cystathionine-b-synthase (CBS) and Nox2 by 3 times and 1.8 times, respectively, after 4 hours in mouse vascular endothelial cell line, UV-2 cell cultured on stress chamber. On the contrary, the extension stress did not induced mRAN expression of eNOS. We examined the effect of the extension stress on the mRNA expression of various redox-related genes in human condroblastic cell line, ATDC5 cell. The extension stress also induced Nox2, iNOS, eNOS, and CBS mRNA expression after 2 to 4 hours, but not cystathionine-v-lyase. These evidences suggested that persistent extension stress induced production of endogenous H2S.

Research Products

• [Journal Article] Gender differences in the association between metabolic syndrome and periodontal disease (2015)
  • Author(s): Furuta, M., Shimazaki1, Y., Takeshita, T., Shibata, Y., Akifusa, F, Eshima, N., Kiyohara, Y., Hirakawa, Y., Mukai, N., Nagata, M, and Yamashita, Y.
  • Journal Title: the Hisayama Study. J Clin Periodontol Volume: 40 Pages: 743-752
  • DOI: 10.1111/jcpe.12119
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] High-level activities of daily living and disease-specific mortality during a 12-year follow-up of an 80-year-old population. (2013)
  • Author(s): Takata Y, Ansai T, Soh I, Awano S, Nakamichi I, Akifusa S, Goto K, Yoshida A, Fujii H, Fujisawa R, Sonoki K
  • Journal Title: Clinical Interventions in Aging Volume: 8 Pages: 721-728
  • DOI: 10.2147/cia.s43480
  • Peer Reviewed
• [Journal Article] Activities of daily living dependency and disease-specific mortality during 12-year follow-up in an 80-year-old population. (2013)
  • Author(s): Takata Y, Ansai T, Soh I, Awano S, Nakamichi I, Akifusa S, Goto K, Yoshida A, Sonoki K
  • Journal Title: Aging Clinical and Experimental Research, ISSN 1720-8319 Aging Clin Exp Res Volume: 25(2) Pages: 193-201
  • DOI: 10.1007/s40520-013-0029-6
  • Peer Reviewed
• [Journal Article] Body mass index and disease-specific mortality in an 80-year-old population at the 12-year follow-up. (2013)
  • Author(s): Takata Y, Ansai T, Soh I, Awano S, Nakamichi I, Akifusa S, Goto K, Yoshida A, Fujii H, Fujisawa R, Sonoki K
  • Journal Title: Archives of Gerontrology and Geriatrics Volume: 57(1) Pages: 46-53
  • DOI: 10.1016/j.archger.2013.02.006
  • Peer Reviewed
• [Journal Article] Relationship between nutrition status and dental occlusion in community-dwelling frail elderly people. (2013)
  • Author(s): Kikutani T, Yoshida M, Enoki H, Yamashita Y, Akifusa S, Shimazaki Y, Hirano H, Tamura F
  • Journal Title: Geriatr Gerontol Int. Volume: 13(1) Pages: 50-54
  • DOI: 10.1111/j.1447-0594.2012.00855.x
  • Peer Reviewed
• [Journal Article] Essential role of JAK/STAT pathway in the induction of cell cycle arrest in macrophages infected with periodontopathic bacterium Aggregatibacter actinomycetemcomitans (2013)
  • Author(s): Okinaga T, Ariyoshi W, Akifusa S, Nishihara T.
  • Journal Title: Medical Microbiology and Immunology Volume: 202 Pages: 167-174
  • DOI: 10.1007/s00430-012-0282-x
  • Peer Reviewed
• [Journal Article] Interrelationship of oral health status, swallowing function, nutritional status, and congnitive ability with activities of daily living in Japanese elderly people receiving home care services due to physical disabilities (2013)
  • Author(s): Furuta M, Komiya-Nonaka M, Akifusa S, Shimazaki Y, Adachi M, Kinoshita T, Kikutani T, Yamashita Y
  • Journal Title: Community Dent Oral Epidemiol. Volume: 41(2) Pages: 173-181
  • DOI: 10.1111/cdoe.12000
  • Peer Reviewed
• [Journal Article] Effectiveness of the Salivary Occult Blood Test as a Screening Method for Periodontal Status (2011)
  • Author(s): Shimazaki, Y., et.al.
  • Journal Title: Journal of Periodontology Volume: 82(4) Pages: 581-587
  • DOI: 10.1902/jop.2010.100304
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase alpha regulates globular adiponectin-induced reactive oxygen species (2011)
  • Author(s): Kamio N, Akifusa S, Yamashita Y
  • Journal Title: Free Radic Res Volume: 45(3) Pages: 336-341
  • DOI: 10.3109/10715762.2010.532495
  • Peer Reviewed
• [Journal Article] Involvement of suppressor of cytokine signaling-1 in globular adiponectin-induced granulocyte colony-stimulating factor in Raw 264 cell Mollcular Immunology (2011)
  • Author(s): Fujimoto A, Akifusa S, Hirofuji T, Yamashita Y
  • Journal Title: Mol Immunol Volume: 48(15-16) Pages: 2052-2858
  • DOI: 10.1016/j.molimm.2011.06.440
  • Peer Reviewed
• [Book] Adiponectin: Production, Regulation and Roles in Disease. (2012)
  • Author(s): Akifusa S, et al.
  • Total Pages: 10
  • Publisher: NOVA Science