Positive feedback loop of Rac activity on synapse structural plasticity
| Project/Area Number |
24680036
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Neuroscience in general
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SANEYOSHI Takeo 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (00556201)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥27,560,000 (Direct Cost: ¥21,200,000、Indirect Cost: ¥6,360,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
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| Keywords | シナプス / アクチン / スパイン / シナプス可塑性 / 長期増強 / アクチン細胞骨格 / Rac / CaMKII / 海馬 / 長期増強現象 |
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| Outline of Final Research Achievements |
In this grant period, we have successfully visualized Rac activation induced by LTP stimulation by two-photon fluorescent lifetime imaging microscopy (2pFLIM). The Rac 2pFLIM revealed that Rac activation lasted more than 30 min post LTP stimulation, which depended on NMDA-receptor, CaMKII, and TIAM1. We also demonstrated that CaMKII was locked in active conformation through interaction with TIAM1 in independently from phosphorylation of Thr-286.
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Report
(4 results)
Research Products
(10 results)
