Positive feedback loop of Rac activity on synapse structural plasticity

• Project/Area Number: 24680036
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SANEYOSHI Takeo 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (00556201)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥27,560,000 (Direct Cost: ¥21,200,000、Indirect Cost: ¥6,360,000); Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2012: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
• Keywords: シナプス / アクチン / スパイン / シナプス可塑性 / 長期増強 / アクチン細胞骨格 / Rac / CaMKII / 海馬 / 長期増強現象

Outline of Final Research Achievements

In this grant period, we have successfully visualized Rac activation induced by LTP stimulation by two-photon fluorescent lifetime imaging microscopy (2pFLIM). The Rac 2pFLIM revealed that Rac activation lasted more than 30 min post LTP stimulation, which depended on NMDA-receptor, CaMKII, and TIAM1. We also demonstrated that CaMKII was locked in active conformation through interaction with TIAM1 in independently from phosphorylation of Thr-286.

Research Products

• [Journal Article] A Naturally Occurring Null Variant of the NMDA Type Glutamate Receptor NR3B Subunit Is a Risk Factor of Schizophrenia (2015)
  • Author(s): Matsuno H, Ohi K, Hashimoto R, Yamamori H, Yasuda Y, Fujimoto M, Yano-Umeda S, Saneyoshi T, Takeda M, Hayashi Y
  • Journal Title: PLoS One Volume: 10(3) Issue: 3 Pages: e0116319-e0116319
  • DOI: 10.1371/journal.pone.0116319
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural and molecular remodeling of dendritic spine substructures during long-term potentiation. (2014)
  • Author(s): Bosch M, Castro J, Saneyoshi T, Matsuno H, Sur M, Hayashi Y.
  • Journal Title: Neuron Volume: 82(2) Issue: 2 Pages: 444-59
  • DOI: 10.1016/j.neuron.2014.03.021
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Synapse reorganization-a new partnership revealed. (2014)
  • Author(s): Saneyoshi T, Hayashi Y.
  • Journal Title: EMBO J. Volume: 33(12) Pages: 1292-4
  • DOI: 10.1002/embj.201488619
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Synapse reorganization--a new partnership revealed. (2014)
  • Author(s): Saneyoshi T, Hayashi Y.
  • Journal Title: EMBO Journal Volume: In press
• [Journal Article] The Ca^<2+> and Rho GTPase signaling pathways underlying activity-dependent actin remodeling at dendritic spines, (2012)
  • Author(s): Saneyoshi T., Hayashi Y.
  • Journal Title: Cytoskeleton Volume: 69(8) Pages: 545-554
  • Peer Reviewed
• [Presentation] Conversion of a transient Ca2+ signaling into a persistent structural modification of dendritic spines by CaMKII/TIAM complex formation during synaptic plasticity. (2015)
  • Author(s): Takeo Saneyoshi
  • Organizer: MDFLI 2015
  • Place of Presentation: 京都 国立京都国際会館
  • Year and Date: 2015-01-26 – 2015-01-28
• [Presentation] Conversion of a transient Ca2+ signaling into a persistent structural modification of dendritic spines by CaMKII/TIAM complex formation during synaptic plasticity. (2014)
  • Author(s): Takeo Saneyoshi, Hitomi Matsuno, Nathan Hedrick, Hideji Murakoshi, Ryohei Yasuda, and Yasunori Hayashi
  • Organizer: SfN 2014
  • Place of Presentation: 米国 ワシントンDC
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Persistent Interaction Between CaMKII and TIAM1 during structural plasticity of single dendritic spines. (2014)
  • Author(s): Takeo Saneyoshi
  • Organizer: FENS 2014
  • Place of Presentation: イタリア ミラノ市
  • Year and Date: 2014-07-05 – 2014-07-09
• [Presentation] Regulation of synapse structural plasticity using photoactivatable signaling proteins (2013)
  • Author(s): Saneyoshi T., Hayashi Y.
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] Persistent Interaction between CaMKII and TIAM1 during long-term potentiation (2013)
  • Author(s): Saneyoshi T., Hayashi Y.
  • Organizer: US-Japan Symposium on Neuronal plasticity
  • Place of Presentation: Seattle, USA