| Budget Amount *help |
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2012: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
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| Outline of Final Research Achievements |
Gene expression analyses of Sphingobium fuliginis OMI, isolated from the rhizosphere of Spirodela polyrrhiza (giant duckweed), were performed using DNA microarray to clarify the mechanism of specific enrichment of this microbe in the rhizosphere of duckweeds.
Although most of the strongly expressed genes of strain OMI were related to metabolisms of substrates and transcriptional regulators, the genes related to synthesize chemotaxis protein and type IV pilus were unique during the symbiotic cultivation with duckweeds. The type IV pilus is reported to act as an anchor to attach to a surface of a certain host. Thus, one of the possible mechanisms for the selective enrichment of strain OMI by duckweeds is the induction of genes to synthesize chemotaxis protein, which allows strain OMI to move to the rhizosphere of duckweeds, and the induction of genes to synthesize type IV pilus, which allows strain OMI to attach onto the root surface of the duckweeds.
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