Highly efficient cell-free membrane protein expression in organic-inorganic hybrid vesicles
| Project/Area Number |
24681028
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Research Field |
Microdevices/Nanodevices
|
|---|
| Research Institution | Nara Institute of Science and Technology |
|---|
Principal Investigator |
Yasuhara Kazuma 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (90545716)
|
|---|
| Project Period (FY) |
2012-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
|
|---|
| Keywords | バイオリアクター / マイクロ・ナノデバイス / 有機－無機ハイブリッド / ベシクル / 遺伝子発現 / 有機ー無機ハイブリッド / 膜タンパク質 / タンパク質 / 有機-無機ハイブリッド / 蛋白質 |
|---|
| Outline of Final Research Achievements |
This study focused on the development of a highly efficient cell-free membrane protein expression system using organic-inorganic hybrid vesicles. The organic-inorganic hybrid vesicles with a crosslinked ceramic-like surface have a semi-permeable feature in which small molecules can permeate across the membrane whereas large molecules cannot. The structure and semi-permeable feature of the organic-inorganic hybrid vesicles can be tuned by the incorporation of various amphiphiles. It was suggested that the encapsulation of enzymatic reaction system enables the development of a membrane protein expression system, which utilizes the semi-permeable feature of the organic-inorganic hybrid vesicles.
|
Report
(5 results)
Research Products
(36 results)
-
-
-
-
-
[Journal Article] Porphyrin-uptake in liposomes and living cells using an exchange method with cyclodextrin2015
Author(s)
A. Ikeda, S. Hino, T. Mae, Y. Tsuchiya, K. Sugikawa, M. Tsukamoto, K. Yasuhara, H. Shigeto, H. Funabashi, A. Kuroda, M. Akiyama
-
Journal Title
RSV Adv.
Volume: 5
Issue: 127
Pages: 105279-105287
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article]2014
Author(s)
S. Junedi, K. Yasuhara, S. Nagao, J. Kikuchi, S. Hirota
-
Journal Title
ChemBioChem
Volume: Vol. 15, No. 4
Issue: 4
Pages: 517-521
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
