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Highly efficient cell-free membrane protein expression in organic-inorganic hybrid vesicles

Research Project

Project/Area Number 24681028
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Microdevices/Nanodevices
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Yasuhara Kazuma  奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (90545716)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Keywordsバイオリアクター / マイクロ・ナノデバイス / 有機-無機ハイブリッド / ベシクル / 遺伝子発現 / 有機ー無機ハイブリッド / 膜タンパク質 / タンパク質 / 有機-無機ハイブリッド / 蛋白質
Outline of Final Research Achievements

This study focused on the development of a highly efficient cell-free membrane protein expression system using organic-inorganic hybrid vesicles. The organic-inorganic hybrid vesicles with a crosslinked ceramic-like surface have a semi-permeable feature in which small molecules can permeate across the membrane whereas large molecules cannot. The structure and semi-permeable feature of the organic-inorganic hybrid vesicles can be tuned by the incorporation of various amphiphiles. It was suggested that the encapsulation of enzymatic reaction system enables the development of a membrane protein expression system, which utilizes the semi-permeable feature of the organic-inorganic hybrid vesicles.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (36 results)

All 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (1 results) Journal Article (12 results) (of which Peer Reviewed: 11 results,  Acknowledgement Compliant: 4 results,  Open Access: 1 results) Presentation (23 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Int'l Joint Research] ミシガン大学(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Anisotropic Self-Assembly of Citrate-Coated Gold Nanoparticles on Fluidic Liposomes2016

    • Author(s)
      K. Sugikawa, T. Kadota, K. Yasuhara, A. Ikeda
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 55 Issue: 12 Pages: 4059-4063

    • DOI

      10.1002/anie.201511785

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Kinetic study of all-or-none hemolysis induced by catonic amphiphilic polymethacrylates with antimicorbial activity2015

    • Author(s)
      Kazuma Yasuhara, Kenichi Kuroda
    • Journal Title

      Chinese Chemical Letters

      Volume: 印刷中 Issue: 4 Pages: 479-484

    • DOI

      10.1016/j.cclet.2015.01.029

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Lipid-Membrane-Incorporated Hydrophobic Photochromic Molecules Prepared by the Exchange Method Using Cyclodextrins2015

    • Author(s)
      A. Ikeda, S. Hino, K. Ashizawa, K. Sugikawa, J. Kikuchi, M. Tsukamoto, K. Yasuhara
    • Journal Title

      Org. Biomol. Chem.

      Volume: 13 Issue: 22 Pages: 0-0

    • DOI

      10.1039/c5ob00240k

    • NAID

      120006469197

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Porphyrin-uptake in liposomes and living cells using an exchange method with cyclodextrin2015

    • Author(s)
      A. Ikeda, S. Hino, T. Mae, Y. Tsuchiya, K. Sugikawa, M. Tsukamoto, K. Yasuhara, H. Shigeto, H. Funabashi, A. Kuroda, M. Akiyama
    • Journal Title

      RSV Adv.

      Volume: 5 Issue: 127 Pages: 105279-105287

    • DOI

      10.1039/c5ra24985f

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] [70]Fullerenes Assists the Formation of Phospholipid Bicelles at Low Lipid Concentrations.2014

    • Author(s)
      A. Ikeda, K. Kiguchi, T. Hida, K. Yasuhara, K. Nobusawa, M. Akiyama, W. Shinoda
    • Journal Title

      Langmuir

      Volume: 30 Issue: 41 Pages: 12315-12320

    • DOI

      10.1021/la503732q

    • NAID

      120006469202

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dynamic behaviour in giant unilamellar vesicles induced by the uptake of [70]fullerene2014

    • Author(s)
      A. Ikeda, T. Hida, T. Iizuka, M. Tsukamoto, J. Kikuchi and K. Yasuhara
    • Journal Title

      Chem. Commun.

      Volume: 50 Issue: 11 Pages: 1288-1291

    • DOI

      10.1039/c3cc47711h

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2014

    • Author(s)
      S. Junedi, K. Yasuhara, S. Nagao, J. Kikuchi, S. Hirota
    • Journal Title

      ChemBioChem

      Volume: Vol. 15, No. 4 Issue: 4 Pages: 517-521

    • DOI

      10.1002/cbic.201300728

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of raft domains in a lipid bilayer by boundary-active curcumin2014

    • Author(s)
      M. Tsukamoto, K. Kuroda, A. Ramamoorthy and K. Yasuhara
    • Journal Title

      Chem. Commun.

      Volume: 50 Issue: 26 Pages: 3427-3430

    • DOI

      10.1039/c3cc47738j

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthetic cell division system: Controlling equal vs. unequal divisions by design2013

    • Author(s)
      Yoichi Sato, et. al.
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 3475-3475

    • DOI

      10.1038/srep03475

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneously Formed Semipermeable Organic-Inorganic Hybrid Vesicles Permitting Molecular Weight Selective Transmembrane Passage2013

    • Author(s)
      K. Yasuhara, T. Kawasaki, S. Okuda, S. Oshima, J. Kikuchi
    • Journal Title

      Chem. Commun

      Volume: 49(7) Issue: 7 Pages: 665-667

    • DOI

      10.1039/c2cc36662b

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] セラミック表面によって安定化された脂質二分子膜ナノディスクの形成2013

    • Author(s)
      安原主馬
    • Journal Title

      C&I commun

      Volume: 38 Pages: 29-32

    • NAID

      40020187323

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Thermal Stability of Synthetic Lipid Bicelles Encompassed by Siloxane Surfaces as Organic-Inorganic Hybrid Nanodiscs2012

    • Author(s)
      K. Yasuhara, H. Hayashi J. Kikuchi
    • Journal Title

      Chem. Lett

      Volume: 41(10) Issue: 10 Pages: 1223-1225

    • DOI

      10.1246/cl.2012.1223

    • NAID

      130004426360

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 両親媒性高分子による脂質膜の断片化とナノディスク形成2016

    • Author(s)
      荒木田臣・菊池純一・安原主馬
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学 京田辺キャンパス(京都府京田辺市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Design of Lipid Molecules for Specific Domain Formation in Artificial Cell Membranes2016

    • Author(s)
      Yuri, Ishikura, Yoshiyuki Horiuchi, Taku Yamazaki,Kazuma Yasuhara, Jun-ichi Kikuchi
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学 京田辺キャンパス(京都府京田辺市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] SPONTANEOUS LIPID NANODISC FORMATION BY MEMBRANE-ACTIVE POLYMERS2016

    • Author(s)
      K. Yasuhara, J. Arakida, J. Kikuchi
    • Organizer
      60th Biophysical Society Annual Meeting
    • Place of Presentation
      Los Angeles Convention Center, Los Angeles, CA, USA
    • Year and Date
      2016-02-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Designed amphiphilic polymers for lipid membrane nanodisc formation2015

    • Author(s)
      K. Yasuhara, J. Arakida, J. Kikuchi
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii Convention Center, Honolulu, HI, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Design and evaluation of membrane-active polymers to form lipid bilayer nanodisc2015

    • Author(s)
      K. Yasuhara, J. Arakida, J. Kikuchi
    • Organizer
      Macromolecular Science & Engineering 39th Annual Symposium
    • Place of Presentation
      University of Michigan, Ann Arbor, MI, USA
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脂質ナノディスクを形成する膜活性ポリマーの設計2015

    • Author(s)
      荒木田臣・菊池純一・安原主馬
    • Organizer
      第64回高分子討論会
    • Place of Presentation
      東北大学 川内キャンパス(宮城県仙台市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Membrane-active amphiphilic polymers for lipid nanodisc formaiton2015

    • Author(s)
      安原主馬・荒木田臣・菊池純一
    • Organizer
      第53回生物物理学会年会
    • Place of Presentation
      金沢大学 角間キャンパス(石川県金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脂質ナノディスクを形成する膜活性ポリマー2015

    • Author(s)
      安原主馬・荒木田臣・菊池純一
    • Organizer
      第66回コロイドおよび界面化学討論会
    • Place of Presentation
      鹿児島大学 郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Membrane-active amphiphilic polymers for lipid nanodisc formation2015

    • Author(s)
      K. Yasuhara, J. Arakida, J. Kikuchi
    • Organizer
      IACIS 2015
    • Place of Presentation
      Convention Center Rheingoldhalle, Mainz, Germany
    • Year and Date
      2015-05-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Self-Assembly of Organic-Inorganic Hybrid Lipids in Water Toward Fabrication of Bio-nanomaterials2014

    • Author(s)
      K. Yasuhara
    • Organizer
      RPI MTLE department seminar
    • Place of Presentation
      Troy, NY / USA
    • Year and Date
      2014-11-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Fablication of semipermeable organic-inorganic hybrid vesicles and their application to nanocapsule for enzymatic reaction2014

    • Author(s)
      K. Yasuhara, J. Kikuchi
    • Organizer
      "The 7th International Workshop on ADVANCED MATERIALS SCIENCE AND NANOTECHNOLOGY "
    • Place of Presentation
      Ha Long City / Vietnam
    • Year and Date
      2014-11-02 – 2014-11-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Interaction of Zinc-Chelating Polymer with Cell Membranes2014

    • Author(s)
      M. Namba, M. Tsukamoto, J. Kikuchi, K. Yasuhara
    • Organizer
      Macromolecular Science & Engineering 38th Annual Symposium
    • Place of Presentation
      Ann Arbor, MI / USA
    • Year and Date
      2014-10-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Organic-inorganic Hybrid Vesicles as Spontaneously Formed Semipermeable Nanocapsule2014

    • Author(s)
      K. Yasuhara, J. Kikuchi
    • Organizer
      XXIII INTERNATIONAL MATERIALS RESEARCH CONGRESS 2014
    • Place of Presentation
      Cancun / Mexico
    • Year and Date
      2014-08-17 – 2014-08-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 有機無機ハイブリッドベシクルの選択的膜透過を利用した酵素反応制御2013

    • Author(s)
      大島匠平・安原主馬・菊池純一
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学(滋賀県)(ポスター発表)
    • Year and Date
      2013-03-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] 有機-無機ハイブリッドベシクルの分子ふるい機能とバイオナノリアクターへの応用2013

    • Author(s)
      安原主馬・大島匠平・菊池純一
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学(滋賀県)
    • Year and Date
      2013-03-23
    • Related Report
      2012 Annual Research Report
  • [Presentation] カチオン性カリックスアレーン誘導体の抗菌活性2013

    • Author(s)
      永井佐知・安原主馬・菊池純一
    • Organizer
      第62回高分子学会年次大会
    • Place of Presentation
      京都国際会館(京都府京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 抗菌性カリックスアレーン誘導体の構造-活性相関2013

    • Author(s)
      永井佐知・安原主馬・菊池純一
    • Organizer
      第7回バイオ関連シンポジウム
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Supontaneously formed semipermeable organic-inorganic hybrid vesicles and their application to bio-nano reactors2013

    • Author(s)
      K. Yasuhara, J. Kikuchi
    • Organizer
      XVII International Sol-Gel Conference
    • Place of Presentation
      Melia Castilla Hotel (Madrid, Spain)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Organic-Inorganic Hybrid Vesicle as Semipermeable Nano-capsule2012

    • Author(s)
      K. Yasuhara, J. Kikuchi
    • Organizer
      The Macro/NAIST Joint Workshop for Emerging Researchers
    • Place of Presentation
      University of Michigan Ann Arbor, MI USA(招待講演)
    • Year and Date
      2012-10-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] 有機-無機ハイブリッドベシクル「セラソーム」における分子量選択的膜透過とその制御2012

    • Author(s)
      安原主馬, 奥田静代, 菊池純一
    • Organizer
      日本ゾル-ゲル学会 第10回討論会
    • Place of Presentation
      慶應義塾大学(東京都)(ポスター発表)
    • Year and Date
      2012-07-27
    • Related Report
      2012 Annual Research Report
  • [Presentation] セラソームの半透膜機能とその制御2012

    • Author(s)
      安原主馬, 奥田静代, 大島匠平, 菊池純一
    • Organizer
      第61回高分子学会年次大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-05-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] 有機-無機ハイブリッド脂質の自己組織化とバイオ応用

    • Author(s)
      安原主馬
    • Organizer
      奈良先端未来開拓コロキウム
    • Place of Presentation
      奈良先端科学技術大学院大学(奈良県生駒市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Antimicrobial activity of amphiphilic calix[4]arene derivatives

    • Author(s)
      S. Nagai, K. Yasuhara, J. Kikuchi
    • Organizer
      Macromolecular Science & Engineering 37th Annual Symposium
    • Place of Presentation
      University of Michigan (Ann Arbor, MI USA)
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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