|Budget Amount *help
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
|Outline of Final Research Achievements
Germline cells are sole source of next generation in multicellular organism, which is called cellular totipoency. Germ cells erase genome-wide DNA methylation and histone modifications to acquire cellular totipotency during their development.
In this study, we have tried to identify the molecular mechanisms and biological significance of base excision repair-mediated active demethyaltion by PRDM14. We have shown that PRDM14 regulates the activity of TET proteins to promotes active DNA demethylation in embryonic stem cells (ESCs). We have further demonstrated that PRDM14 converts epiblast-like cells (EpiLCs) to ESCs mediated by the enhancement of OCT3/4-binding at target locus through active DNA demethylation.