| Budget Amount *help |
¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Outline of Final Research Achievements |
In this study, I challenged the development of chiral-isomer specific isotope analysis of nitrogen, carbon, and hydrogen within amino acids. The results show that the isotope analysis of nitrogen and carbon is successfully achieved by using a combination of pivaloyl/isobuthyl ester derivatization with a polar GC column, resulting in a 1 sigma standard deviation being better than ± 0.5‰ based on the S/N ratio of >20 with baseline separation on the chromatogram. I also briefly confirmed that this method is widely applicable to biological (e.g., animal tissues), geological (e.g., sendiments), and extraterrestrial samples (meteorites). In contrast, the isotope analysis of hydrogen is still in the early-development stage, because I cannot control the considerable isotopic fractionation of hydrogen/deuterium on the amino group of amino acids during extraction and GC elution processes.
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