Biological functions of ultradian oscillation revealed by bioluminescence and fluorescence imaging

Research Project

Project/Area Number	24700293
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Bioinformatics/Life informatics
Research Institution	Kyoto University
Principal Investigator	ISOMURA Akihiro 京都大学, ウイルス研究所, 研究員 (70512466)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	システム生物学 / 光遺伝学 / 定量生物学 / 細胞・組織 / 生体生命情報学
Outline of Final Research Achievements	We tought to visualize genetic oscillation and myogenic differentiation process simultaneously in myoblast cells by combining fluorescence and bioluminescence imaging tequniques. We succeeded in the visualization of Hes1 oscillatory dynamics in myogenic differentiation, but we did not find correlation between ultradian dynamics and differentiation states. On the other hand, we also developed infrared fluorecent reporter of cell cycle progression and optogenetic tools to control gene expression dynamics in a precise temporal manner.

Report

(4 results)