Biological functions of ultradian oscillation revealed by bioluminescence and fluorescence imaging
| Project/Area Number |
24700293
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | システム生物学 / 光遺伝学 / 定量生物学 / 細胞・組織 / 生体生命情報学 |
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| Outline of Final Research Achievements |
We tought to visualize genetic oscillation and myogenic differentiation process simultaneously in myoblast cells by combining fluorescence and bioluminescence imaging tequniques. We succeeded in the visualization of Hes1 oscillatory dynamics in myogenic differentiation, but we did not find correlation between ultradian dynamics and differentiation states. On the other hand, we also developed infrared fluorecent reporter of cell cycle progression and optogenetic tools to control gene expression dynamics in a precise temporal manner.
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Report
(4 results)
Research Products
(4 results)
