Genetic and cellular mechanisms generating sexually dimorphic neuronal structures and behavior in Drosophila

• Project/Area Number: 24700309
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: Tohoku University
• Principal Investigator: Sato Kosei 東北大学, 生命科学研究科, 助教 (40451611)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ショウジョウバエ / 性行動 / 脳 / 性差 / fruitless / RNAスプライシング / ユビキチン / BTB/POZ / 神経 / 転写因子 / Fruitless / クロマチン / ユビキチン化 / 遺伝子 / 発生 / 行動 / RNA

Outline of Final Research Achievements

The Drosophila gene fruitless produces male-specific Fruitless, which orchestrates the expression of ~100 target genes, although their transcription is not always sex-specific. This study shows that Fruitless produces sex differences in a target gene product Lola by blocking its proteolysis; males with Fruitless acquire full-length Lola whereas females acquire a Lola proteolytic product, and both Lola products induce distinct sex-specific neural characteristics. Fruitless transcribes lola, protects Lola from proteolysis and forms a complex with Lola for transcriptional regulation. This triad of interactions control neuronal sexual differentiation underlying gendered behavior in Drosophila.

Research Products

• [Journal Article] Zeste tunes the timing of ecdysone actions in triggering programmed tissue degeneration in Drosophila (2015)
  • Author(s): Goto T., Sato K., Sone H., Koganezawa M., Ito H., Yamamoto D.
  • Journal Title: J. Neurogenet. Volume: 29 Issue: 4 Pages: 169-173
  • DOI: 10.3109/01677063.2015.1098638
  • Peer Reviewed
• [Journal Article] A fruitless upstream region that defines the species specificity in the male-specific muscle patterning in Drosophila. (2014)
  • Author(s): Takayanagi, S., Toba, G., Lukacsovich, T., Ote, M., Sato, K., and Yamamoto, D.
  • Journal Title: J. Neurogenet. Volume: 29 Issue: 1 Pages: 23-29
  • DOI: 10.3109/01677063.2014.983101
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The Drosophila Lingerer protein cooperates with Orb2 in long-term memory formation. (2014)
  • Author(s): Kimura, S., Sakakibara, Y., Sato, K., Ote, M., Ito, H., Koganezawa, M., and Yamamoto, D.
  • Journal Title: J. Neurogenet. Volume: 29 Issue: 1 Pages: 8-17
  • DOI: 10.3109/01677063.2014.917644
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] An epigenetic switch of the brain sex as a basis of gendered behavior in Drosophila. (2014)
  • Author(s): Sato K. and Yamamoto D.
  • Journal Title: Advances in Genetics Volume: 86 Pages: 45-63
  • DOI: 10.1016/b978-0-12-800222-3.00003-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 「越境する性」の生物学 (Biological bases for typical and atypical gendered behaviors) (2014)
  • Author(s): 山元大輔, 佐藤耕世
  • Journal Title: 岩波 科学 Volume: 84 (7) Pages: 736-744
• [Journal Article] The Drosophila Lingerer protein cooperates with Orb2 in long-term memory formation. (2014)
  • Author(s): Kimura S., Sakakibara Y., Sato K., Ote M., Ito H., Koganezawa M. and Yamamoto D.
  • Journal Title: Journal of Neurogenetics Volume: accepted
  • Peer Reviewed
• [Journal Article] Neuroethology of male courtship in Drosophila: from the gene to behavior. (2014)
  • Author(s): Yamamoto D., Sato K., and Koganezawa M.
  • Journal Title: J. Comp. Physiol. A. Volume: 200 Issue: 4 Pages: 251-264
  • DOI: 10.1007/s00359-014-0891-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The female brain and the male brain. (2013)
  • Author(s): Yamamoto D., Sato K.
  • Journal Title: Brain and Nerve Volume: 65 (10) Pages: 1147-1158
  • NAID: 40019825578
• [Journal Article] Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. (2012)
  • Author(s): Ito, H., Sato, K., Koganezawa, M., Ote, M., Matsumoto, K., Hama, C., Yamamoto, D.
  • Journal Title: Cell Volume: 149 Issue: 6 Pages: 1327-1338
  • DOI: 10.1016/j.cell.2012.04.025
  • Peer Reviewed
• [Journal Article] Fruitlessは拮抗する二つのクロマチン制御因子と共同して単一ニューロンレベルの性的二型を作り出す (2012)
  • Author(s): 佐藤耕世
  • Journal Title: 東北大学クロスオーバー Volume: 第15号 Pages: 4-5
• [Presentation] Searching for fru-target genes that regulate the development of sexual dimorphisms in Drosophila central neurons. (ポスター発表) (2015)
  • Author(s): Ito H., Sato K., Kondo S., Ueda R. and Yamamoto D.
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際展示場（兵庫県・神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Searching for fru-target genes that regulate the development of sexual dimorphisms in Drosophila central neurons. (口頭発表) (2015)
  • Author(s): Ito H., Sato K., Kondo S., Ueda R. and Yamamoto D.
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Searching for fru-target genes that regulate the development of sexual dimorphisms in Drosophila central neurons. (2015)
  • Author(s): Ito H., Sato K., Kondo S., Ueda R. and Yamamoto D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2015-11-25
• [Presentation] Sex-specific cleavage of Lola specifies sex-specific neurite structures in Drosophila. (2015)
  • Author(s): Sato K., Ito H., Koganezawa M., Toba G. and Yamamoto D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2015-11-25
• [Presentation] Male-specific transcription factor Fruitless non-transcriptionally suppresses proteolytic cleavage of a Lola isoform to generate sexual differences in neuronal structures and behavior of Drosophila. (2015)
  • Author(s): Sato K., Ito H., Koganezawa M. and Yamamoto D.
  • Organizer: 2015 CSHL Meeting on Neurobiology of Drosophila
  • Place of Presentation: Cold Spring Harbor Laboratory, NY, U.S.A.
  • Year and Date: 2015-09-29
• [Presentation] Trf2 may act as a co-factor of Fruitless, a neural masculinizing factor. (2014)
  • Author(s): Chowdury ZS., Sato K. and Yamamoto D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: 宮城蔵王
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] A possible involvement of non-cell-autonomous mechanism in shaping sexually dimorphic neuronal structures. (2014)
  • Author(s): Kato T., Sato K. and Yamamoto D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: 宮城蔵王
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] A genetic screen for Fru-target genes required for sexually-dimorphic projection patterning in Drosophila neurons. (2014)
  • Author(s): Ito H., Sato K., Kondo S. and Yamamoto D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: 宮城蔵王
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Sex-specific functions of longitudinals lacking. (2014)
  • Author(s): Sato K., Toba G., Ito H., Koganezawa M., Yamamoto D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: 宮城蔵王
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] ショウジョウバエ脳神経系の雄化因子Fruitlessがもつ転写調節以外の機能 (2014)
  • Author(s): 佐藤耕世, 鳥羽岳太, 伊藤弘樹, 小金澤雅之, 山元大輔
  • Organizer: 新学術領域研究「性差構築の分子基盤」第6回領域会議
  • Place of Presentation: 浜松
  • Year and Date: 2014-10-09 – 2014-10-11
• [Presentation] A potential non-transcriptional role of Fruitless that specifies neuronal sex-types and fly sexual orientation. (2014)
  • Author(s): Sato K., Toba G., Ito H., Koganezawa M., Yamamoto D.
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-11 – 2014-09-14
• [Presentation] A possible role for the CPEB-interacting protein in the long-term memory formation explored in Drosophila melanogaster. (2013)
  • Author(s): Kimura S., Sunouchi K., Sato K., Ito H., Nomoto S., Sakakibara Y. and Yamamoto D.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Sex-specific functions of longitudinals lacking. (2013)
  • Author(s): Sato K., Toba G., Ito H., Koganezawa M., Yamamoto D.
  • Organizer: 2013 CSHL Meeting on Neurobiology of Drosophila
  • Place of Presentation: NY, U.S.A.
• [Presentation] Non-cell-autonomous mechanism generates sexually dimorphic neuronal structures in the Drosophila brain. (2013)
  • Author(s): Kato T., Sato K., Yamamoto D.
  • Organizer: Asia-Pacific Drosophila Research Conference 2013
  • Place of Presentation: Seoul, Korea
• [Presentation] Non-cell-autonomous mechanism generates sexually dimorphic neuronal structures in the Drosophila brain. (2013)
  • Author(s): Kato T., Sato K., Yamamoto D.
  • Organizer: NIH-Tohoku University-JSPS Symposium
  • Place of Presentation: 仙台
• [Presentation] Non-cell-autonomous mechanism generates sexually dimorphic neuronal structures in the Drosophila brain. (2013)
  • Author(s): Kato T, Sato K, Yamamoto D.
  • Organizer: The 4th UCL-Tohoku University Symposium
  • Place of Presentation: London, UK
• [Presentation] ショウジョウバエのクロマチン制御因子Zesteは変態時に起こる細胞死を組織に応じて正または負に制御する (2012)
  • Author(s): 後藤貴章、佐藤耕世、伊藤弘樹、山元大輔
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、福岡
• [Remarks] 東北大学大学院生命科学研究科脳機能遺伝分野
  • URL: http://www.biology.tohoku.ac.jp/lab-www/yamamoto_lab/index.html
• [Remarks] Kosei Sato Web Page
  • URL: https://sites.google.com/site/koseisato777/top-page
• [Remarks] 東北大学大学院生命科学研究科脳機能遺伝分野（山元大輔研究室）
  • URL: http://www.biology.tohoku.ac.jp/lab-www/yamamoto_lab/
• [Remarks] 東北大学大学院生命科学研究科脳機能遺伝分野
  • URL: http://www.biology.tohoku.ac.jp/lab-www/yamamoto_lab/
• [Remarks] Kosei Sato Web Page
  • URL: https://sites.google.com/site/koseisato777/
• [Remarks] Kosei Sato Web Page
  • URL: https://sites.google.com/site/koseisato777/top-page