Selective transduction into parallel fiber or climbing fiber using antibody-displaying viral vector
| Project/Area Number |
24700394
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Gunma University |
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Principal Investigator |
KONNO Ayumu 群馬大学, 医学(系)研究科(研究院), 助教 (40509048)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ウイルスベクター / 小脳 / 抗体 |
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| Outline of Final Research Achievements |
The aim of this research is to establish the method for a selective transduction into parallel fiber or climbing fiber in a mouse cerebellum using an antibody-displaying viral vector. To achieve this aim, we made new antibodies against lumenal domain of VGluT1 or VGluT2. We injected the lentiviral vectors displaying these antibodies into mice cerebellum. However, these vectors could not infect specific cells that were expected. To achieve this aim, we need a different approaches such as adeno-associated virus vector.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Mutant ataxin-3 with an abnormally expanded polyglutamine chain disrupts dendritic development and metabotropic glutamate receptor signaling in mouse cerebellar purkinje cells2014
Author(s)
Konno A, Shuvaev AN, Miyake N, Miyake K, Iizuka A, Matsuura S, Huda F, Nakamura K, Yanagi S, Shimada T, Hirai H.
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Journal Title
Cerebellum
Volume: 13
Issue: 1
Pages: 29-41
DOI
Related Report
Peer Reviewed
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