Analysis of physical properties of actin filaments during differentiation of smooth muscle cells

• Project/Area Number: 24700454
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: The University of Kitakyushu (2013); Osaka University (2012)
• Principal Investigator: KIHARA Takanori 北九州市立大学, 国際環境工学部, 准教授 (90436535)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: CRP2 / アクチン繊維 / 平滑筋細胞 / 構造 / 物性 / X線小角散乱 / 原子間力顕微鏡 / 共焦点顕微鏡 / 繊維の物性 / 脱重合

Research Abstract

Dynamics of actin filaments regulate differentiation of smooth muscle cells. In this study, I examined the effects of CRP2, which is a specific transcription cofactor for smooth muscle cell related genes, on structure and physical property of actin filaments. The structure of CRP2 in solution was demonstrated using small angle X-ray scattering analysis. CRP2 increased the diameter and the persistent length of the actin filaments by binding with actin filaments. Furthermore, the mechanical properties of smooth muscle cells were increased by overexpression of CRP2. Therefore, I clarified that CRP2 was a factor regulating the structure and physical property of actin filaments in solution.

Research Products

• [Journal Article] Measurement of biomolecular diffusion in extracellular matrix condensed by fibroblasts using fluorescence correlation spectroscopy (2013)
  • Author(s): Kihara, T., Ito, J., Miyake, J
  • Journal Title: PLoS ONE Volume: 8 Issue: 11 Pages: e82382-e82382
  • DOI: 10.1371/journal.pone.0082382
  • Peer Reviewed
• [Journal Article] Fabrication of in vitro three-dimensional multilayered blood vessel model using human endothelial and smooth muscle cells and high-strength PEG hydrogel (2013)
  • Author(s): Shinohara S, Kihara T, Sakai S, Matsusaki M, Akashi M, Taya M, Miyake J
  • Journal Title: J Biosci Bioeng Volume: 116 Issue: 2 Pages: 231-4
  • DOI: 10.1016/j.jbiosc.2013.02.013
  • NAID: 110009657208
  • Peer Reviewed
• [Journal Article] Elasticity mapping analysis of apical cell periphery actin structures of normal fibroblasts and cervical cancer cells (2013)
  • Author(s): Kihara T, Yoshida T, Haghparast S.M.A, Miyake, J
  • Journal Title: J. Analytical Sciences Volume: 3 Issue: 02 Pages: 124-9
  • DOI: 10.4236/jasmi.2013.32015
  • Peer Reviewed
• [Presentation] Smooth muscle differentiation related transcription factor CRP2 directly regulates of actin filaments dynamics (2013)
  • Author(s): Kihara T., Shinohara S., Shinohara S., Sugimoto Y., Miyake, J.
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都
• [Presentation] 細胞内外における分子拡散解析 (2013)
  • Author(s): 伊東潤里,木原隆典,山崎淳平,三宅淳
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島
• [Presentation] 細胞骨格リモデリング過程における細胞の力学解析 (2013)
  • Author(s): 吉田拓士, 木原隆典, Haghparast SMA, 三宅淳
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島
• [Presentation] Elastic and cytoskeletal phenotype of floating cancer cells (2013)
  • Author(s): Haghparast, S.M.A., Kihara, T., Yoshida, T., Miyake, J.
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島
• [Presentation] Mechanostructural features of cervical cancer cells in adhesion states (2013)
  • Author(s): Haghparast, S.M.A., Kihara, T., Shimizu, Y., Miyake, J.
  • Organizer: The 69th Annual Meeting of the Japanese Society of Microscopy
  • Place of Presentation: 大阪
• [Presentation] 細胞のバイオメカニクス (2012)
  • Author(s): 木原隆典,清水祐司, Haghparast SMA,三宅淳
  • Organizer: 日本機械学会第23回バイオフロンティア講演会
  • Place of Presentation: 弘前
• [Presentation] Smooth muscle differentiation related transcription factor CRP2 directly regulates physical properties of actin filaments (2012)
  • Author(s): Kihara T., Shinohara S., ugimoto Y., Miyake, J.
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
• [Presentation] 細胞のバイオメカニクス (2012)
  • Author(s): 木原隆典, 清水祐司, Haghparast SMA, 三宅淳
  • Organizer: 日本機械学会 第23回バイオフロンティア講演会
  • Place of Presentation: 弘前
• [Presentation] 細胞のバイオメカニクス (2012)
  • Author(s): 木原隆典, Haghparast SMA, 清水祐司, 三宅淳
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸
• [Presentation] ゼラチンゲルを基質として用いた血管様構造体の構築 (2012)
  • Author(s): 篠原翔, 木原隆典, 境慎司, 田谷正仁, 三宅淳
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸
• [Presentation] Fabrication of in vitro blood vessel model using PEG crosslinking gelatin hydrogel (2012)
  • Author(s): 篠原翔, 木原隆典, 境慎司, 田谷正仁, 三宅淳
  • Organizer: 生体医工学シンポジウム2012
  • Place of Presentation: 豊中
• [Book] Regulation of differentiated phenotypes of vascular smooth muscle cells , Current Basic and Pathological Approaches to the Function of Muscle Cells and Tissues - From Molecules to Humans, Sugi, H. (ed) (2012)
  • Author(s): Shinohara, S., Shinohara, S., Kihara, T., Miyake, J.
  • Publisher: InTech
• [Book] Current Basic and Pathological Approaches to the Function of Muscle Cells and Tissues -From Molecules to Humans (2012)
  • Author(s): Shinohara, S., Shinohara, S., Kihara, T., Miyake, J.
  • Publisher: InTech