Single bout of running exercise suppressed autophagy in the fasted state

• Project/Area Number: 24700705
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: Juntendo University
• Principal Investigator: ZHENG DONGMEI 順天堂大学, 医学(系)研究科(研究院), 非常勤助教 (10420829)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: Autophagy / Endurance exercise / LC3 / muscle / mTOR / treadmill / autophagy / endurance exercise / オートファジー / 運動 / 筋肉 / S6

Research Abstract

Starvation-induced decrease in insulin and serum amino acids effectively suppresses the mammalian target of rapamycin (mTor) signaling to induce autophagy, a cellular major degradative pathway, in skeletal muscles (soleus, plantaris, and gastrocnemius). I investigated effect of treadmill running exercise on mTor signaling of skeletal muscles of starved mice. I found that mTor signaling pathway once inactivated under starvation conditions was reactivated after treadmill running exercise (12 m/min, 2 h) as revealed by the transition of phosphorylation state of S6-kinase and ribosomal S6. In accordance with this, autophagosomes induced in the skeletal muscles of starved GFP-transgenic mice were markedly suppressed after exercise. Treadmill running exercise caused changes in the expression of glycogenin, galectin-1, etc. However, these changes do not seem to connect to the mechanism of exercise-induced mTor reactivation.

Research Products

• [Journal Article] Ferulic acid induces mammalian target of rapamycin inactivation in cultured mammalian cells (2013)
  • Author(s): Bian Z, Furuya N, Zheng DM, Oliva Trejo JA, Tada N, Ezaki J, Ueno T
  • Journal Title: Biol Pharm Bull Volume: 36 Pages: 120-124
• [Journal Article] Ferulic Acid Induces Mammalian Target of Rapamycin Inactivation in Cultured Mammalian Cells (2013)
  • Author(s): Zehua, B. et al.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 36 Issue: 1 Pages: 120-124
  • DOI: 10.1248/bpb.b12-00695
  • NAID: 130001869764
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Interleukin-11 links oxidative stress and compensatory proliferation (2012)
  • Author(s): Nishina T, Komazawa-Sakon S, Yanaka S, Piao X, Zheng DM, Piao JH, Kojima Y, Yamashina S, Sano E, Putoczki T, Doi T, Ueno T, Ezaki J, Ushio H, Ernst M, Tsumoto K, Okumura K, Nakano H
  • Journal Title: Sci Signal Volume: 5
• [Journal Article] Interleukin-11 Links Oxidative Stress and Compensatory Proliferation (2012)
  • Author(s): Nishina T, Komazawa-Sakon S, Yanaka S, Zheng X, Palo J, Kojima Y, Yamashina S, Sano E, Putoczki T, Doi T, Ueno T, Ezaki J, Ushio H, Ernst M, Kouhei T, Okamura K, Nakano H
  • Journal Title: Science Signal Volume: 5 Issue: 207
  • DOI: 10.1126/scisignal.2002056
  • Peer Reviewed
• [Journal Article] Autophagy is suppressed in soleus and plantaris muscles by endurance exercise. (2012)
  • Author(s): Zheng DM, Bian Z, Furuya N, Takeda-Ezaki M, Oliva Trejo JA, Takahashi K, Hiraoka Y, Fujimura T, Komatsu M, Kominami E, Ueno T, Ezaki J.
  • Journal Title: Spotology Volume: 1 Pages: 189-192