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Development of novel assay systems for evaluating food components and detecting their physiological effects through Drosophila disease models

Research Project

Project/Area Number 24700837
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits, studies on eating habits
Research InstitutionYamanashi Gakuin Junior College (2014-2015)
University of Shizuoka (2012-2013)

Principal Investigator

Kayashima Yasunari  山梨学院短期大学, その他部局等, 准教授 (90365453)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords食情報 / ショウジョウバエ / 食品機能 / 実験動物 / 疾患モデル生物 / 機能性評価 / 国際情報交換
Outline of Final Research Achievements

In this study, we established the novel use of Drosophila as an experimental organism for analyzing the physiological functions of food components. We analyzed the physiological effects of actual food components on antioxidative functions and energy metabolism systems, which are mechanisms Drosophila shares with humans. Furthermore, we assembled a series of mutant lineages to serve as Drosophila disease models for investigating potential relationships between food components and gene functions, which overcome many of the problems and disadvantages of the currently available assay systems that utilize mice, rats, and cultured cells. These lineages also allow for rapid detection of the genes and gene pathways affected by food components, as well as enable the detection of the effects of food components in whole organisms.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2015 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Tea polyphenols ameliorate fat storage induced by high-fat diet in Drosophila melanogaster.2015

    • Author(s)
      Kayashima Y., Murata S., Sato M., Matsuura K., Asanuma T., Chimoto J., Ishii T., Mochizuki K., Kumazawa S., Nakayama T., Yamakawa-Kobayashi K
    • Journal Title

      Biochem. Biophys. Reports

      Volume: 4 Pages: 417-424

    • DOI

      10.1016/j.bbrep.2015.10.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Autocrine regulation of ecdysone synthesis by β3-octopamine receptor in the prothoracic gland is essential for Drosophila metamorphosis.2015

    • Author(s)
      Ohhara Y., Shimada-Niwa Y., Niwa R., Kayashima Y., Hayashi Y., Akagi K., Ueda H., Yamakawa-Kobayashi K., and Kobayashi S.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 112 Issue: 5 Pages: 1452-1457

    • DOI

      10.1073/pnas.1414966112

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A heteroallelic Drosophila insulin-like receptor mutant and its use in validating physiological activities of food constituents.2013

    • Author(s)
      Kayashima Y., Sato A., Kumazawa S., Yamakawa-Kobayashi K.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 434 Issue: 2 Pages: 258-262

    • DOI

      10.1016/j.bbrc.2013.02.113

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of a Dipeptide-Enriched Diet in an Adult <i>Drosophila melanogaster</i> Laboratory Strain2013

    • Author(s)
      Shiotani S., Yanai N., Suzuki T., Tujioka S., Sakano Y., Yamakawa-Kobayashi K., Kayashima Y.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 4 Pages: 836-838

    • DOI

      10.1271/bbb.120606

    • NAID

      10031170727

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of Prx3, a Drosophila ortholog of the thiol-dependent peroxidase PRDX3, in age-dependent oxidative stress resistance2012

    • Author(s)
      Kayashima Y., Yamakawa-Kobayashi K.
    • Journal Title

      Biomed. Res.

      Volume: 33 Pages: 319-322

    • NAID

      130004903835

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Freeze-dried royal jelly maintains its developmental and physiological bioactivity in Drosophila melanogaster2012

    • Author(s)
      Kayashima Y, Yamanashi K, Sato A, Kumazawa S, Yamakawa-Kobayashi K.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 76 Pages: 2107-2111

    • NAID

      10031126516

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] モデル生物ショウジョウバエを用いた生活習慣病関連遺伝子の解析ならびに機能性食品成分の探索2015

    • Author(s)
      萱嶋泰成
    • Organizer
      山梨学院学術報告会
    • Place of Presentation
      山梨学院生涯学習センター
    • Year and Date
      2015-02-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 食品の機能性検定に向けた二型糖尿病モデルショウジョウバエの開発と利用2014

    • Author(s)
      萱嶋 泰成, 佐藤 綾香, 熊澤 茂則, 小林 公子
    • Organizer
      公益社団法人日本動物学会第84回大会
    • Place of Presentation
      東北大学
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Regulation of energy metabolism by carnosine dipeptidase (CNDP)2014

    • Author(s)
      Yamakawa-Kobayashi K., Otagi E., Sakano Y., Shiotani S., Yanai N., Kayashima Y.
    • Organizer
      International Congress on Carnosine and Anserine 2014 (ICCA 2014)(国際学会)
    • Place of Presentation
      Hoshi University
    • Year and Date
      2014-08-07
    • Related Report
      2014 Research-status Report
  • [Presentation] Effect of Carnosine and Anserine-Enriched Diet in Fruit Fly Drosophila melanogaster.2014

    • Author(s)
      Kayashima Y., Shiotani S., Yanai N., Suzuki T., Tujioka S., Sakano Y., Yamakawa-Kobayashi K.
    • Organizer
      International Congress on Carnosine and Anserine 2014 (ICCA 2014)(国際学会)
    • Place of Presentation
      Hoshi University
    • Year and Date
      2014-08-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 茶ポリフェノールがもつ脂肪蓄積の抑制効果 -モデル生物ショウジョウバエによる検証-2014

    • Author(s)
      萱嶋 泰成, 村田 晋一, 佐藤 美咲, 青木 あずさ, 地本 純子, 小林 公子
    • Organizer
      第29回茶学術研究会講演会
    • Place of Presentation
      ホテルアソシア静岡(静岡県)
    • Related Report
      2013 Research-status Report
  • [Presentation] カルノシン分解酵素(CNDP)とエネルギー代謝の関連2013

    • Author(s)
      小林 公子, 小田木 絵璃, 坂野 ゆりえ, 萱嶋 泰成
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場 (兵庫県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 茶ポリフェノールの生理機能をショウジョウバエで解析する試み2013

    • Author(s)
      萱嶋 泰成, 村田 晋一, 佐藤 美咲, 地本 純子, 小林 公子
    • Organizer
      公益社団法人日本動物学会第84回大会
    • Place of Presentation
      岡山大学 (岡山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] テアフラビンとエピテアフラガリンの生理活性作用 -ショウジョウバエを用いた検証-2012

    • Author(s)
      萱嶋 泰成, 村田 晋一, 小林 公子
    • Organizer
      第17回日本フードファクター学会学術集会・第9回日本カテキン学会総会 合同大会
    • Place of Presentation
      静岡県男女共同参画センター・あざれあ(静岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] ショウジョウバエインスリン受容体の新規突然変異体の解析2012

    • Author(s)
      佐藤 綾香, 萱嶋 泰成, 小林 公子
    • Organizer
      第17回日本フードファクター学会学術集会・第9回日本カテキン学会総会 合同大会
    • Place of Presentation
      静岡県男女共同参画センター・あざれあ(静岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] キイロショウジョウバエで探る茶ポリフェノールの抗酸化、抗老化作用2012

    • Author(s)
      村田 晋一, 萱嶋 泰成, 小林 公子
    • Organizer
      第17回日本フードファクター学会学術集会・第9回日本カテキン学会総会 合同大会
    • Place of Presentation
      静岡県男女共同参画センター・あざれあ(静岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] カルノシン分解酵素CNDP1のショウジョウバエ相同遺伝子dCNDP1の機能2012

    • Author(s)
      萱嶋 泰成, 坂野 ゆりえ, 辻岡 志穂, 塩谷 茂信, 柳内 延也, 小林 公子
    • Organizer
      公益社団法人日本動物学会第83回大会
    • Place of Presentation
      大阪大学 (大阪府)
    • Related Report
      2012 Research-status Report
  • [Presentation] モデル動物ショウジョウバエによる 茶ポリフェノールの生理機能解析

    • Author(s)
      地本 純子, 村田 晋一, 佐藤 美咲, 萱嶋 泰成, 小林 公子
    • Organizer
      第28回茶学術研究会講演会
    • Place of Presentation
      ホテルアソシア静岡 (静岡県)
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 老化抑制剤2012

    • Inventor(s)
      萱嶋 泰成, 中山 勉, 片柳 悠紀, 今井 伸二郎
    • Industrial Property Rights Holder
      萱嶋 泰成, 中山 勉, 片柳 悠紀, 今井 伸二郎
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-05-30
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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