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Newly vaccine strategy with mAbs against heat shock protein 90 (HSP90) on surface of antigen presenting cell (APC)

Research Project

Project/Area Number 24700992
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor immunology
Research InstitutionOkayama University

Principal Investigator

SHUSAKU Mizukami  岡山大学, 医歯(薬)学総合研究科, 助教 (00508971)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords熱ショックタンパク質 / 抗体 / ワクチン / 抗原提示細胞 / アジュバント / モノクローナル抗体 / 細胞膜表面 / heat shock protein / クロスプレゼンテーション
Research Abstract

Although HSP90 is a cytosolic molecular chaperone, our originally produced mAb 6H8 could detect cell surface HSP90 on APCs. As so far we reported, injection of 6H8 chemically conjugated with Ag (6H8-Ag) induced proliferation of adoptively transferred Ag specific CD8+T cells efficiently, compared with free Ag. Importantly those proliferation was dramatically enhanced by co-administration of free 6H8.
So I planned a project to confirm this phenomenon with solid tumor and human Ag model. But we found that 6H8's affinity was affected by Fc portion, due to it was an IgG2a Ab. Then, we prepared another cell surface HSP90 detectable IgG1 mAb and modified IgG1 type 6H8. Besides, we start immunization for Abs against STIP1 and HSC70, component of cell surface HSP complex in our hypothesis. Although, we couldn't carry out our original project enough so far, already mouse model for experiments with human Ag is available, so after finish the preparation of those Abs, we can restart our project.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2013 2012 Other

All Presentation (8 results)

  • [Presentation] 抗原提示細胞膜表面hsp90を標的としたmAbを用いたワクチン開発の基盤的研究2013

    • Author(s)
      水上修作, 他
    • Organizer
      第17回日本がん免疫学会学術集会・総会
    • Place of Presentation
      山口
    • Related Report
      2013 Final Research Report
  • [Presentation] Newly vaccine strategy with mAbs against heat shock protein 90 (HSP90) on surface of antigen presenting cell (APC)2013

    • Author(s)
      水上修作, 他
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗原提示細胞膜表面HSP90を標的としたmAbを用いたワクチン開発の基盤的研究2013

    • Author(s)
      水上修作、山﨑千尋、岡山容子、鵜殿平一郎
    • Organizer
      第17回 日本がん免疫学会総会
    • Place of Presentation
      ANAクラウンプラザホテル宇部
    • Related Report
      2013 Annual Research Report
  • [Presentation] Newly vaccine strategy with mAbs against heat shock protein 90 (HSP90) on surface of antigen presenting cell (APC).2013

    • Author(s)
      水上修作、岡山容子、山崎千尋、鵜殿平一郎
    • Organizer
      第72回 日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] 抗原提示細胞膜表面hsp90を標的としたmAbを用いたワクチン開発の基盤的研究2012

    • Author(s)
      水上修作, 他
    • Organizer
      第16回日本がん免疫学会学術集会・総会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗原提示細胞膜表面hsp90を標的としたmAbを用いたワクチン開発の基盤的研究2012

    • Author(s)
      水上修作, 他
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗原提示細胞膜表面hsp90を標的としたmAbを用いたワクチン開発の基盤的研究

    • Author(s)
      水上修作、山崎千尋、鵜殿平一郎
    • Organizer
      第16回日本がん免疫学会総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] 抗原提示細胞膜表面hsp90を標的としたmAbワクチン開発の基盤的研究

    • Author(s)
      水上修作、山崎千尋、鵜殿平一郎
    • Organizer
      第71回 日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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