Development of high performance cell separation and label-free cell detection methods by biomimetic methods
| Project/Area Number |
24710135
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Osaka University (2013-2014)
Nagoya University (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 循環腫瘍細胞 / マイクロ流体デバイス / 非侵襲分離 / 非標識検出 / 抽出 / CTC / 非標識 / バイオミメティック / プロテアーゼ |
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| Outline of Final Research Achievements |
I successfully developed less-invasive and easy cell separation, and label-free cell detection methods for circulating tumor cells (CTCs). Because current methods have severe damage on cells and require troublesome procedures in cell separation and detection, present results would make it possible to accelerate medical research about CTCs by separation and culturing CTCs. Furthermore, for improving cell separation method and an extraction process for intracellular molecules such as DNA and RNA, a new combined method was developed for cell separation and extraction. This developed method makes it easy to collect cells and extract intracellular molecules such as DNA and RNA, and would decrease sample loss during extraction. Therefore, a developed method is expected to be applied for cell separation and characterization of rare cells such as CTC.
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Report
(4 results)
Research Products
(11 results)
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[Book] 現代化学2013
Author(s)
岡本行広 馬場嘉信
Total Pages
5
Publisher
東京化学同人
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