Study on new metabolisms in plant vacuole by integration of the post-genome analysis

• Project/Area Number: 24710235
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied genomics
• Research Institution: Kobe University
• Principal Investigator: OHNISHI Miwa 神戸大学, 理学(系)研究科(研究院), 助教 (10437501)
• Research Collaborator: YAMAMOTO Kotaro 神戸大学 ; ANEGAWA Aya 神戸大学 ; MIMURA Tetsuro 神戸大学 ; FUKAKI Hidehiro 神戸大学 ; ISHIZAKI Kimitsune 神戸大学 ; SHICHIJO Chizuko 神戸大学 ; MURAKAMI Akio 神戸大学 ; MASUJIMA Tsutomu 理化学研究所 ; MIZUNO Hajime 理化学研究所 ; TAKAHASHI Katsutoshi 産業技術総合研究所 ; YAMAZAKI Mami 千葉大学
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 液胞 / ニチニチソウ / 二次代謝 / ポストゲノム解析

Outline of Final Research Achievements

Vacuole is an important organelle in the plant cell. It occupies about 80% of the plant cell volume. It is known that a variety of substances are accumulated in the vacuole. In this study, we focused on the biosynthesis of alkaloids that are accumulated in the vacuole of Catharanthus roseus, this is one of famous medical plants. From previous studies, alkaloids have been shown to be synthesized through various tissues and organelles. We analyzed the localization of alkaloids and their intermediates by using new technology. There are some different results from previous reports. These results suggest the existence of novel metabolic functions in the vacuole of Catharanthus roseus. In the future, we would like to apply the present new knowledge to the development of efficient alkaloid biosynthesis system.

Research Products

• [Journal Article] Involvement of Ca2+ in vacuole degradation caused by a rapid temperature decrease in Saintpaulia palisade cells: A case of gene expression analysis in a specialized small tissue. (2015)
  • Author(s): Ohnishi M., Kadohama N., Suzuki Y., Kajiyama T., Shichijo C., Ishizaki K., Fukaki H., Iida H., Kambara H., Mimura T.
  • Journal Title: Plant & Cell Physiology Volume: 印刷中 Issue: 7 Pages: 1297-1035
  • DOI: 10.1093/pcp/pcv048
  • Peer Reviewed / Open Access
• [Journal Article] Induced accumulation of glucuronosyldiacylglycerol in tomato and soybean under phosphorus deprivation. (2015)
  • Author(s): Okazaki Y., Nishizawa T., Takano K., Ohnishi M., Mimura T., Saito K.
  • Journal Title: Physiologia Platarum Volume: 印刷中 Issue: 1 Pages: 33-42
  • DOI: 10.1111/ppl.12334
  • Peer Reviewed
• [Journal Article] Altered levels of primary metabolites in response to exogenous indole-3-acetic acid in wild type and auxin signaling mutants of <i>Arabidopsis thaliana</i>: A capillary electrophoresis-mass spectrometry analysis (2015)
  • Author(s): Anegawa A., Ohnishi M., Takagi D., Miyake C., Shichijo C., Ishizaki K., Fukaki H., Mimura T.
  • Journal Title: Plant Biotechnology Volume: 32 Issue: 1 Pages: 65-79
  • DOI: 10.5511/plantbiotechnology.15.0116a
  • NAID: 130005061610
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Autoradiography of Pi Distribution in Barley Seedlings. (2014)
  • Author(s): Kanno S., Kurita Y., Ohnishi M., Mimura, T.
  • Journal Title: Bio-protocol Volume: -
  • Peer Reviewed
• [Journal Article] Extraction of Ions from Leaf Sections. (2014)
  • Author(s): Kurita Y., Kanno S., Ohnishi M., Mimura, T.
  • Journal Title: Bio-protocol Volume: -
  • Peer Reviewed
• [Journal Article] Establishment of a shortened annual cycle system; a tool for the analysis of annual re-translocation of phosphorus in the deciduous woody plant (Populus alba L.). (2014)
  • Author(s): Kurita Y., Baba K., Ohnishi M., Anegawa A., Shichijo C., Kosuge K., Fukaki H., Mimura T.
  • Journal Title: Journal of Plant Research Volume: 127 Issue: 4 Pages: 545-551
  • DOI: 10.1007/s10265-014-0634-2
  • NAID: 40020110959
  • Peer Reviewed
• [Journal Article] Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley. (2013)
  • Author(s): Nagai Makiko, Ohnishi Miwa, Uehara Takeo, Yamagami Mutsumi, Miura Eiko, Kamakura Mai, Kitamura Akira, Sakaguchi Shuichi, Sakamoto Wataru, Shimmen Teruo, Fukaki Hidehiro, Reid Rob, J., Furukawa Akio and Mimura Tetsuro
  • Journal Title: Plant, Cell & Environment Volume: 36 Issue: 10 Pages: 1826-1837
  • DOI: 10.1093/pcp/pct107
  • Peer Reviewed
• [Journal Article] Sudden collapse of vacuoles in Saintpaulia sp. palisade cells induced by a rapid temperature decrease. (2013)
  • Author(s): Kadohama N, Goh T, Ohnishi M, Fukaki H, Mimura T, Suzuki Y.
  • Journal Title: PLoS One Volume: 8(2)
  • NAID: 120005465542
  • Peer Reviewed
• [Journal Article] Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley. (2013)
  • Author(s): Nagai M., Ohnishi M., Uehara T., Yamagami M., Miura E., Kamakura M., et al.
  • Journal Title: Plant, Cell and Environment Volume: 36 Issue: 10 Pages: 1826-1837
  • DOI: 10.1111/pce.12090
  • Peer Reviewed
• [Presentation] 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム (2015)
  • Author(s): 大西美輪, 角浜憲明, 鈴木祥弘, 梶山智晴, 七條千津子, 石崎公庸, 深城英弘, 飯田秀利, 神原秀記, 三村徹郎
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ニチニチソウ茎組織におけるTerpenoid indole alkaloidの分布解析 (2015)
  • Author(s): 山本浩太郎, 大西美輪, 姉川彩, 高橋勝利, 水野初, 七條千津子, 石崎公庸, 山崎真巳, 深城英弘, 升島努, 三村徹郎
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Mechanism of cell injury induced by a rapid temperature decrease in Saintpaulia sp. leaves. (2015)
  • Author(s): Ohnishi M., Kadohama N., Suzuki Y., Kajiyama T., Shichijo C., Ishizaki K., Fukaki H., Iida H., Kambara H., and Mimura T.
  • Organizer: The 2nd International Symposium on Plant Environmental Sensing
  • Place of Presentation: AIST, Odaiba
  • Year and Date: 2015-03-13 – 2015-03-15
• [Presentation] Cell-specific localization of terpenoid indole alkaloids revealed by new metabolome analyses (2015)
  • Author(s): Yamamoto K., Ohnishi M., Anegawa A., Takahashi K., Mizuno H., Shichijo C., Ishizaki K., Yamazaki M., Fukaki H., Masujima T., Mimura T.
  • Organizer: The 2nd International Symposium on Plant Environmental Sensing
  • Place of Presentation: AIST, Odaiba
  • Year and Date: 2015-03-13 – 2015-03-15
• [Presentation] Novel metabolome analyses of terpenoid indole alkaloids synthesis in Catharanthus roseus (2014)
  • Author(s): Yamamoto K., Ohnishi M., Anegawa A., Takahashi K., Iwasaki T., Mizuno H., Yamazaki M., Masujima T., Mimura T.
  • Organizer: THE 38th NAITO CONFERENCE ON Molecule-based biological systems
  • Place of Presentation: CHATERAISE Gateaux Kingdom SAPPORO, Hokkaido, Japan
  • Year and Date: 2014-10-07 – 2014-10-10
• [Presentation] 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム (2014)
  • Author(s): 大西美輪, 角浜憲明, 高橋勝利, 七條千津子, 石崎公庸, 深城英弘, 鈴木祥弘, 三村徹郎
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] ニチニチソウ組織におけるTerpenoid indole alkaloid合成機構の解明 (2014)
  • Author(s): 山本浩太郎, 大西美輪, 姉川彩, 高橋勝利, 岩崎哲史, 水野初, 七條千津子, 石崎公庸, 山崎真巳, 深城英弘, 升島努, 三村徹郎
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム (2014)
  • Author(s): 大西美輪, 角浜憲明, 高橋大輔, 七條千津子, 石崎公庸, 深城英弘, 上村松生, 鈴木祥弘, 三村徹郎
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] ニチニチソウ葉組織の単一細胞種を用いた二次代謝機構の解析 (2013)
  • Author(s): 山本浩太郎, 大西美輪, 姉川彩, 高橋勝利, 岩崎哲史, 水野初, 七條千津子, 石崎公庸, 山崎真巳, 深城英弘, 升島努, 三村徹郎
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学
• [Presentation] 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム (2013)
  • Author(s): 大西美輪, 角浜憲明, 七條千津子, 石崎公庸, 深城英弘, 鈴木祥弘, 三村徹郎
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学
• [Presentation] 植物液胞のポストゲノム解析
  • Author(s): 大西美輪
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫県立大学
• [Presentation] ニチニチソウ細胞における二次代謝産物合成機構の解析
  • Author(s): 山本浩太郎
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫県立大学
• [Presentation] ニチニチソウはどうやって抗がん剤を作るのか？：二次代謝における細胞間／細胞内調節
  • Author(s): 山本浩太郎
  • Organizer: 第３回サイエンスフロンティア研究発表会
  • Place of Presentation: 神戸大学
• [Presentation] 二次代謝の理解から応用研究へ
  • Author(s): 山本浩太郎
  • Organizer: 若手フロンティア研究会２０１２
  • Place of Presentation: 神戸大学
• [Presentation] ニチニチソウ細胞における二次代謝機能の解析
  • Author(s): 山本浩太郎
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学