Research on the target identification and mechanism analysis of low molecular weight compound

• Project/Area Number: 24710244
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Living organism molecular science
• Research Institution: University of Tsukuba
• Principal Investigator: NAGUMO YOKO 筑波大学, 生命環境系, 助教 (70373339)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 分子標的探索 / 生理活性物質 / 生物分子科学 / 小分子化合物 / 標的分子 / 非共有結合 / 作用機構

Research Abstract

The target identification and mechanism validation of a small compound is important but difficult step. To improve methods concerned, we utilized the antibody, which can recognize a variety of compound structures. We prepared anti-low molecular weight compound antibodies and applied it to target detections.

Research Products

• [Journal Article] Terpendole E and its derivative inhibit STLC- and GSK-1-resistant Eg5 (2014)
  • Author(s): Yuka TARUI, Takumi CHINEN, Yoko NAGUMO, Takayuki MOTOYAMA, Toshiaki HAYASHI, Hiroshi HIROTA, Makoto MUROI, Yasuyuki ISHII, Hisae KONDO, Hiroyuki OSADA, Takeo USUI
  • Journal Title: ChemBioChem Volume: 15 Pages: 934-938
• [Journal Article] Terpendole E and its derivative inhibit STLC - and GSK-1-resistant Eg5 (2014)
  • Author(s): Tarui Y, Chinen T, Nagumo Y, Motoyama T, Hayashi T, Hirota H, Muroi M, Ishii Y, Kondo H, Osada H, Usui T.
  • Journal Title: ChemBioChem Volume: (In press) Issue: 7 Pages: 934-938
  • DOI: 10.1002/cbic.201300808
  • Peer Reviewed
• [Journal Article] The Reversible Increase in Tight Junction Permeability Induced by Capsaicin is Mediated via Cofilin-Actin Cytoskeletal Dynamics and Decreased Level of Occludin (2013)
  • Author(s): Tomoko Shiobara, Takeo Usui, Junkyu Han, Hiroko Isoda, Yoko Nagumo
  • Journal Title: PLOS ONE Volume: 8巻 Issue: 11 Pages: e79954-e79954
  • DOI: 10.1371/journal.pone.0079954
  • Peer Reviewed
• [Journal Article] Glaziovianin A prevents endosome maturation via inhibiting microtubule dynamics (2013)
  • Author(s): Takumi CHINEN, Sayaka KAZAMI, Yoko NAGUMO, Ichiro HAYAKAWA, Akiyuki IKEDO, Masatoshi TAKAGI, Akihito YOKOSUKA, Naoko IMAMOTO, Yoshihiro MIMAKI, Hideo KIGOSHI, Hiroyuki OSADA, Takeo USUI
  • Journal Title: ACS Chem. Biol Volume: 8 Pages: 884-889
• [Journal Article] Glaziovianin A Prevents Endosome Maturation via Inhibiting Microtubule Dynamics. (2013)
  • Author(s): Chinen T, Kazami S, Nagumo Y, Hayakawa I, Ikedo A, Takagi M, Yokosuka A, Imamoto N, Mimaki Y, Kigoshi H, Osada H, Usui T.
  • Journal Title: ACS Chemical Biology Volume: (In press) Issue: 5 Pages: 884-889
  • DOI: 10.1021/cb300641h
  • Peer Reviewed
• [Journal Article] Defining the molecular response to trastuzumab, pertuzumab and combination therapy in ovarian cancer (2012)
  • Author(s): Andrew SIMS, Annelien ZWEEMER, Yoko NAGUMO, Dana FARATIAN, Morwenna MUIR, Michael DODDS, Inhwa UM, Charlene KAY, Max HASMANN, David HARRISON, Simon LANGDON
  • Journal Title: British. J. Cancer Volume: 106 Pages: 1779-1789
• [Journal Article] Defining the molecular response to trastuzumab, pertuzumab and combination therapy in ovarian cancer (2012)
  • Author(s): Andrew Sims, Annelien Zweemer, Yoko Nagumo, Dana Faratian, Morwenna Dodds, Morwenna Muir Inhwa Um, Charlene Kay, Max Hasmann, David Harrison, Simon Langdon
  • Journal Title: British Journal of Cancer Volume: 106 Issue: 11 Pages: 1779-1779
  • DOI: 10.1038/bjc.2012.176
  • Peer Reviewed
• [Presentation] Mode of action of anti-HER2 antibodies in ovarian cancer (2013)
  • Author(s): Yoko NAGUMO, Andrew SIMS, Annelien ZWEEMER, Dana FARATIAN, David HARRISON, Simon LANGDON
  • Organizer: 日本薬学会大133年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2013-03-30
• [Presentation] Capsaicinによる可逆的タイトジャンクション開口メカニズムの解析 (2013)
  • Author(s): 塩原智子, 臼井健郎, 韓畯奎, 礒田博子, 南雲陽子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2013-03-25
• [Presentation] Extacting Various Functions from Food Ingredients : Case Study about Capsaicin (2012)
  • Author(s): Yoko NAGUMO, Junkyu HAN, Hiroko ISODA
  • Organizer: Tunisia-Japan Symposium on Science, Society and Technology (TJASSST)
  • Place of Presentation: Hammamet (Tunisia)
  • Year and Date: 2012-11-17
• [Presentation] Mode of action of anti-HER2 antibodies in ovarian cancer xenograft
  • Author(s): Yoko Nagumo, Sims Andrew, Zweemer Annelien, Dana Faratian, David Harrison, Simon Langdon
  • Organizer: 日本薬学会第１３３年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Capsaicinによる可逆的タイトジャンクション開口メカニズムの解析
  • Author(s): 塩原 智子、臼井 健郎、韓 駿奎、礒田 博子、南雲 陽子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 仙台、東北大学
• [Presentation] Extracting Various Functions from Food Ingredients: Case Study about Capsaicin
  • Author(s): Yoko NAGUMO, Junkyu HAN and Hiroko ISODA
  • Organizer: Tunisia-Japan Symposium on Society, Sciences, and Technology (TJASSST)
  • Place of Presentation: Hammamet, Tunisia
• [Remarks]
  • URL: http://www.agbi.tsukuba.ac.jp/~usui/study/top.html
• [Remarks] 筑波大学 臼井研究室
  • URL: http://www.agbi.tsukuba.ac.jp/~usui/study/top.html