| Project/Area Number |
24710264
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Tokyo Institute of Technology (2013)
Kanagawa University (2012) |
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Principal Investigator |
OKAMOTO Itaru 東京工業大学, 生命理工学研究科, 東工大特別研究員 (40460133)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | RNA検出 / ボロン酸 / 蛍光 / 核酸 / フェニルボロン酸 / 核酸化学 / ボロン酸エステル / 光誘起電子移動 / 分子認識 / プローブ / ジオール |
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| Research Abstract |
The aim of this study is to develop new RNA detecting probe which consisting of a DNA strand with a fluorescing group and a phenyl boronic acid residue. The DNA strand of the probe binds to a 3'-end sequence of a target RNA to form a double helical structure, then the boronic acid residue of the probe and a diol group at the 3'- end of target RNA form boronic esters which results increasement of fluorescence intensity by cancelling photo-induced electron transfer (PET) between the fluorescent group and an amino group to which the phenyl boronic acid group attached.
We successfully synthesized novel RNA detecting probe having a fluorescing group and phenyl boronic acid residue. When the probe formed a duplex with a target RNA, increasement of fluorescence intensity was observed. On the other hand, in the presence of a target DNA strand, increasement of fluorescence intensity was not observed.
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