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Development of self-luminescent inorganic nanosheet/enzyme complex for bioimaging

Research Project

Project/Area Number 24750072
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionSaga University

Principal Investigator

SOH Nobuaki  佐賀大学, 農学部, 准教授 (90336008)

Research Collaborator KAMADA Kai  長崎大学, 大学院工学研究科, 准教授 (90315284)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords無機ナノシート / 酵素 / 発光 / 蛍光 / バイオ材料
Outline of Final Research Achievements

Novel hybrid materials ((i) a hybrid material composed of fluorescent inorganic nanosheet and enzyme (ii) a hybrid material composed of fluorescent compound, non-fluorescent inorganic nanosheet, and enzyme) were developed for imaging application. Europium-doped layered titanates show fluorescent properties and the fluorescence intensity increased with an increase in the concentration of doped europium. Fluorescence derived from chemiluminescent resonance energy transfer (CRET) was observed when luminol derivative was applied to a hybrid material composed of fluorescent beads, horseradish peroxidase (HRP), layered titanates.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (11 results) (of which Invited: 2 results)

  • [Journal Article] Enhanced visible-light-induced photocatalytic activity of alpha-Fe2O3 adsorbing redox enzymes2015

    • Author(s)
      Kai Kamada, Nobuaki Soh
    • Journal Title

      J. Asian Ceram. Soc.

      Volume: 3 Issue: 1 Pages: 18-21

    • DOI

      10.1016/j.jascer.2014.10.004

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Preparation of an enzyme/inorganic nanosheet/magnetic bead complex and its enzymatic activity2014

    • Author(s)
      Nobuaki Soh, Shunsuke Kaneko, Kazuhiro Uozumi, Toshihisa Ueda, Kai Kamada
    • Journal Title

      J. Mater. Sci.

      Volume: 49 Issue: 23 Pages: 8010-8015

    • DOI

      10.1007/s10853-014-8508-5

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Temperature-controlled reversible exfoliationstacking of titanate nanosheets in an aqueous solution containing tetraalkylammonium ions2014

    • Author(s)
      Kai Kamada, Nobuaki Soh
    • Journal Title

      RSC Adv.

      Volume: 4 Issue: 17 Pages: 8682-8685

    • DOI

      10.1039/c3ra47233g

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Soft surface modification of layered titanate for biorecognition2012

    • Author(s)
      Suguru Tsukahara, Nobuaki Soh, Kai Kamada
    • Journal Title

      J. Phys. Chem. C

      Volume: 116 Issue: 36 Pages: 19285-19289

    • DOI

      10.1021/jp304107b

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Visible-light-driven enzymatic reaction of peroxidase adsorbed on doped hematite thin films2012

    • Author(s)
      Kai Kamada, Ayano Moriyasu, Nobuaki Soh
    • Journal Title

      J. Phys. Chem. C

      Volume: 116 Issue: 39 Pages: 20694-20699

    • DOI

      10.1021/jp3046559

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 酵素-層状セラミックス複合体の合成と融合機能2012

    • Author(s)
      鎌田 海, 宗 伸明
    • Journal Title

      化学工業

      Volume: 10 Pages: 39-42

    • Related Report
      2012 Research-status Report
  • [Presentation] 蛍光性チタン酸ナノシートを用いた特定細胞認識2015

    • Author(s)
      德永素子・鎌田 海・宗 伸明
    • Organizer
      日本セラミックス協会2015年年会
    • Place of Presentation
      岡山県岡山市
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 層状鉄チタン酸と結合したペルオキシダーゼの可視光誘起酵素反応2015

    • Author(s)
      伊藤大樹・鎌田 海・宗 伸明
    • Organizer
      日本セラミックス協会2015年年会
    • Place of Presentation
      岡山県岡山市
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 無機ナノシートあるいはメソポーラス炭素材料を利用したグルコースバイオセンサーの開発2014

    • Author(s)
      大島亮太・上田敏久・宗 伸明・鎌田 海
    • Organizer
      2014年度日本農芸化学会西日本支部大会
    • Place of Presentation
      佐賀県佐賀市
    • Year and Date
      2014-09-18 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 無機ナノシートに固定化された酵素を利用した化学発光共鳴エネルギー移動2014

    • Author(s)
      後藤大輝・八尋和也・上田敏久・宗 伸明・鎌田 海
    • Organizer
      第51回化学関連支部合同九州大会
    • Place of Presentation
      福岡県北九州市
    • Year and Date
      2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 酵素固定化材料として無機ナノシートを利用したグルコースバイオセンサーの開発

    • Author(s)
      大島 亮太, 上田 敏久, 宗 伸明, 鎌田 海
    • Organizer
      第50回化学関連支部合同九州大会
    • Place of Presentation
      福岡県北九州市
    • Related Report
      2013 Research-status Report
  • [Presentation] 無機ナノシート/酵素/磁気ビーズ複合体の開発と機能評価

    • Author(s)
      魚住 和弘, 上田 敏久, 宗 伸明, 鎌田 海
    • Organizer
      第50回化学関連支部合同九州大会
    • Place of Presentation
      福岡県北九州市
    • Related Report
      2013 Research-status Report
  • [Presentation] イメージング応用を指向した発光性を有する無機ナノシート/酵素複合体の開発

    • Author(s)
      藤井 佐保, 上田 敏久, 宗 伸明, 鎌田 海
    • Organizer
      第50回化学関連支部合同九州大会
    • Place of Presentation
      福岡県北九州市
    • Related Report
      2013 Research-status Report
  • [Presentation] Layered Iron-titanate as a Physicochemically Stable Support for Hydrolase

    • Author(s)
      Takaharu IKEDA, Suguru TSUKAHARA, Nobuaki SOH, Kai KAMADA
    • Organizer
      4th International Congress on Ceramics
    • Place of Presentation
      Chicago, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Fluorescent Inorganic Titanate Layers as Bioaffinity Reaction Markers

    • Author(s)
      Kai KAMADA, Suguru TSUKAHARA, Nobuaki SOH
    • Organizer
      International Symposium on Rare Earths 2012 in Okinawa for the 30th Anniversary of The Rare Earth Society of Japan
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Fluorescent Compounds for Sensing Biomolecules

    • Author(s)
      Nobuaki SOH
    • Organizer
      Joint Symposium of Saga University and Chonnam National University
    • Place of Presentation
      Saga, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 生体分子を計測する機能性蛍光化合物

    • Author(s)
      宗 伸明
    • Organizer
      長崎大学未来工学研究センターキックオフ研究発表会
    • Place of Presentation
      長崎市
    • Related Report
      2012 Research-status Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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