Development of high-precision quantitative techniques of sequence-specific nucleic acid molecule
| Project/Area Number |
24750079
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
FUJII Shin-ichiro 独立行政法人産業技術総合研究所, 物質計測標準研究部門, 主任研究員 (10415739)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 核酸 / 塩基配列 / 定量分析 / 質量分析 / 分離分析 |
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| Outline of Final Research Achievements |
A mass spectrometry technique for the direct quantification of a nucleic acid molecule of the polymer had saved the nucleotide sequence was performed in this research subject.
We realized the direct quantification of a high-molecular nucleic acid using combining techniques of separation such as high-performance liquid chromatography (HPLC) or capillary electrophoresis and inductively coupled plasma mass spectrometry (ICP-MS). The phosphorus in the molecule of nucleic acid was measured by these analytical methods. Especially, in the method using size exclusion chromatography (SEC) hyphenated with ICP-MS, it was possible to quantify the RNA molecules of 500 to 1000 bases. These results showed the effectiveness and practicality of this approach.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Separation and Quantification of RNA Molecules Using Size-Exclusion Chromatography Hyphenated with Inductively Coupled Plasma-Mass Spectrometry2014
Author(s)
Fujii,S., Inagaki, K., Miyashita, S., Groombridge, A. S., Nagasawa, K., Chiba, K., Takatsu, A.
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Journal Title
ELECTROPHORESIS
Volume: 35
Issue: 9
Pages: 1315-1318
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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