| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
A mass spectrometry technique for the direct quantification of a nucleic acid molecule of the polymer had saved the nucleotide sequence was performed in this research subject.
We realized the direct quantification of a high-molecular nucleic acid using combining techniques of separation such as high-performance liquid chromatography (HPLC) or capillary electrophoresis and inductively coupled plasma mass spectrometry (ICP-MS). The phosphorus in the molecule of nucleic acid was measured by these analytical methods. Especially, in the method using size exclusion chromatography (SEC) hyphenated with ICP-MS, it was possible to quantify the RNA molecules of 500 to 1000 bases. These results showed the effectiveness and practicality of this approach.
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