Development of culture method for evaluation of contractile capability of human skeletal muscle cells
Project/Area Number |
24760650
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biofunction/Bioprocess
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Research Institution | Osaka University |
Principal Investigator |
NAGAMORI Eiji 大阪大学, 工学(系)研究科(研究院), 講師 (70394898)
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Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | 骨格筋 / 筋芽細胞 / 繊維芽細胞 / 張力測定 / 細胞シート / 棲み分け / 自己組織化 / 遊走 / コラーゲン |
Research Abstract |
To enhance active tension capability of human skeletal muscle myotubes derived from human skeletal muscle myoblast population, addition of human skeletal muscle fibroblast cells to myoblast population was examined. Contrary to our expectation, significant improvement of contractile capability of human skeletal muscle myotubes was not achieved, which may be caused by spatial habitation of fibroblast cells in the myoblast tissue due to the differences in its behavior of migration and cell-cell connection. Fibroblast cells mixed in the myoblast tissue rapidly and linearly migrated in the vertical direction and distributed in upper position. Further studies investigating the habitation pattern of various cell types will shed light on controlling the spatial arrangement of the heterogeneous cell population and constructing complicated tissues.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Spatial habitation of heterologous cells in a multi-layered myoblast sheet due to the differences in their behaviors of migration and cell-cell connection2014
Author(s)
Nagamori, E., Oda, M., Nakamura, T., Shimizu, T., Okano, T., Kino-oka, M.
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Journal Title
Cuurent Nanoscience
Volume: 10
Pages: 173-178
Related Report
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[Journal Article] In vitro models for angiogenesis research : a review
Author(s)
Ngo, T.X., Nagamori, E., Shimizu, T., Okano, T., Taya, M., Kino-oka, M.
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Journal Title
International Journal of Tissue Regeneration
Volume: (in press)
Related Report
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