| Project/Area Number |
24770051
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
TAMADA Yosuke 基礎生物学研究所, 生物進化研究部門, 助教 (50579290)
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| Research Collaborator |
HASEBE Mitsuyasu 基礎生物学研究所, 生物進化研究部門, 教授 (40237996)
LI Chen 総合研究大学院大学, 生命科学研究科, 大学院生
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | リプログラミング / RNA / 植物 / 発生 |
|---|
| Research Abstract |
PpCSP, an RNA-binding protein, facilitates the reprogramming from differentiated leaf cells to stem cells in the moss Physcomitrella patens. To reveal the molecular mechanisms underlying the promotion of reprogramming by PpCSP, we have tried to identify the target RNA molecules and the interacting proteins of PpCSP. We performed RNA immunoprecipitation-sequencing and found that PpCSP binds to almost all mRNAs. We previously isolated multiple genes, which exhibited higher transcripts accumulation in the over-expression line of PpCSP than in wild type. We produced over-expression lines of those genes, and found that several over-expression lines exhibited abnormal morphologies. In addition, we isolated multiple interacting partners of PpCSP by performing immunoprecipitation followed by liquid chromatography-mass spectrometry. We confirmed that the cellular localization largely overlapped with that of PpCSP.
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