Crystal structure of Tric channel
Project/Area Number |
24770089
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | The University of Tokyo |
Principal Investigator |
KUBOTA Keiko 東京大学, 分子細胞生物学研究所, 特任研究員 (50597870)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | X線結晶構造解析 / 膜タンパク質 / イオンチャネル |
Research Abstract |
Ca ions are important second messenger in many cellular signal transduction pathways. Trimeric intracellular cation (Tric) channels is thought to play an essential role in Ca-release control from intracellular stores. Furthermore, Tric channel represents a novel class of trimeric monovalent cation channel, especially behaves as K ion channel. To elucidate the structure basis of the Ca-release control mechanism, TricA was expressed in SF9 cells and E coli, and purified and crystallized. We have succeeded overexpression and purification of various Tric homologs. And we obtained the crystals of Chlamydomonas Tric and two type bacteria Trics. Especially, the crystals of SSO greatly grew up and diffracted X-rays to 7.5-angstrom resolution.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Purification, crystallization and preliminary X-ray analysis of OsAREB8 from rice, a member of the AREB/ABF family of bZIP transcription factors, in complex with its cognate DNA.2012
Author(s)
Miyazono, K., Koura, T., Kubota, K., Yoshida, T., Fujita, Y., Yamaguchi-Shinozaki, K., Tanokura, M.
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Journal Title
Acta Cryst. Sect. F
Volume: 68
Issue: 4
Pages: 491-494
DOI
Related Report
Peer Reviewed
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