Crystal structure of Tric channel

• Project/Area Number: 24770089
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KUBOTA Keiko 東京大学, 分子細胞生物学研究所, 特任研究員 (50597870)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: X線結晶構造解析 / 膜タンパク質 / イオンチャネル

Research Abstract

Ca ions are important second messenger in many cellular signal transduction pathways. Trimeric intracellular cation (Tric) channels is thought to play an essential role in Ca-release control from intracellular stores. Furthermore, Tric channel represents a novel class of trimeric monovalent cation channel, especially behaves as K ion channel. To elucidate the structure basis of the Ca-release control mechanism, TricA was expressed in SF9 cells and E coli, and purified and crystallized. We have succeeded overexpression and purification of various Tric homologs. And we obtained the crystals of Chlamydomonas Tric and two type bacteria Trics. Especially, the crystals of SSO greatly grew up and diffracted X-rays to 7.5-angstrom resolution.

Research Products

• [Journal Article] Get1 stabilizes an open dimer conformation of get3 ATPase by binding two distinct interfaces. (2012)
  • Author(s): Kubota K, Yamagata A, Sato Y, Goto-Ito S, Fukai S.
  • Journal Title: J Mol Biol. Volume: 422(3) Issue: 3 Pages: 366-75
  • DOI: 10.1016/j.jmb.2012.05.045
  • Peer Reviewed
• [Journal Article] Molecular basis of Lys-63-linked polyubiquitination inhibition by the interaction between human deubiquitinating enzyme OTUB1 and ubiquitin-conjugating enzyme UBC13. (2012)
  • Author(s): Sato Y, Yamagata A, Goto-Ito S, Kubota K, Miyamoto R, Nakada S, Fukai S.
  • Journal Title: J Biol Chem. Volume: 287(31) Issue: 31 Pages: 25860-8
  • DOI: 10.1074/jbc.m112.364752
  • Peer Reviewed
• [Journal Article] The crystal structure of an intermediate dimer of aspergilloglutamic peptidase that mimics the enzyme-activation product complex produced upon autoproteolysis (2012)
  • Author(s): Sasaki H, Kubota K, Lee WC, Ohtsuka J, Kojima M, Iwata S, Nakagawa A, Takahashi K, Tanokura M
  • Journal Title: J Biochem Volume: 152 Issue: 1 Pages: 45-52
  • DOI: 10.1093/jb/mvs050
  • NAID: 10031166113
  • Peer Reviewed
• [Journal Article] Purification, crystallization and preliminary X-ray analysis of OsAREB8 from rice, a member of the AREB/ABF family of bZIP transcription factors, in complex with its cognate DNA. (2012)
  • Author(s): Miyazono, K., Koura, T., Kubota, K., Yoshida, T., Fujita, Y., Yamaguchi-Shinozaki, K., Tanokura, M.
  • Journal Title: Acta Cryst. Sect. F Volume: 68 Issue: 4 Pages: 491-494
  • DOI: 10.1107/s1744309112009384
  • Peer Reviewed
• [Presentation] Get1-Get3複合体のX線結晶構造解析 (2013)
  • Author(s): ○窪田恵子、 山形敦史、 佐藤裕介、 伊藤（後藤）桜子、 深井周也
  • Organizer: 第30回 PFシンポジウム
  • Place of Presentation: つくば国際会議場（エポカルつくば）
• [Remarks]
  • URL: http://www.iam.u-tokyo.ac.jp/srro/SRROLifeSciDivJp2/Welcome.html