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Generation mechanism of free oligosaccharides in the lumen of the endoplasmic reticulum

Research Project

Project/Area Number 24770134
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

HARADA Yoichiro  独立行政法人理化学研究所, 糖鎖代謝学研究チーム, 特別研究員 (80464147)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords糖鎖代謝 / オリゴ糖転移酵素 / ドリコール結合型糖鎖 / 糖鎖生物学 / 遊離糖鎖
Research Abstract

In this study, I identified an enzyme involved in the metabolism of glycans in the lumen of the endoplasmic reticulum. Asparagine (N)-linked glycosylation is one of the posttranslational modifications, which modulates protein folding and degradation. The enzyme that mediates N-glycosylation is called oligosaccharyltransferase (OST). This enzyme transfers dolichol-linked oligosaccharides (DLOs) onto the specific asparagine residue of proteins. This study uncovered that OST also mediates a hydrolysis of DLOs in budding yeast and mammalian cells.This enzymatic activity has been known for over 30 years, but the responsible enzyme and its biological function were unknown. This study established the molecular basis of the DLO degradation, and opens an new avenue for understanding the biological functions of this novel glycan metabolic pathway.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (10 results) (of which Invited: 2 results) Book (2 results)

  • [Journal Article] Metabolically programmed quality control system for dolichol-linked oligosaccharides2013

    • Author(s)
      Harada Y, Nakajima K, Masahara-Negishi Y, Freeze HH, Angata T, Taniguchi N, Suzuki T
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 110 Issue: 48 Pages: 19366-19371

    • DOI

      10.1073/pnas.1312187110

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Eukaryotic oligosaccharyltransferase generates free oligosaccharides during N-glycosylation2013

    • Author(s)
      Harada Y, Buser R, Ngwa EM, Hirayama H, Aebi M, Suzuki T
    • Journal Title

      J Biol Chem

      Volume: 288 Issue: 45 Pages: 32673-32684

    • DOI

      10.1074/jbc.m113.486985

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] ドリコールオリゴ糖の品質管理機構の同定2013

    • Author(s)
      原田陽一郎、中嶋和紀、根岸由紀、安形高志、谷口直之、鈴木匡
    • Organizer
      第86回日本生化学会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] ドリコールオリゴ糖の品質管理機構2013

    • Author(s)
      原田陽一郎、中嶋和紀、根岸由紀、安形高志、谷口直之、鈴木匡
    • Organizer
      第32回日本糖質学会
    • Place of Presentation
      大阪、大阪国際交流センター
    • Related Report
      2013 Final Research Report
  • [Presentation] The generation of free oligosaccharides in eukaryotes2013

    • Author(s)
      Yoichiro Harada
    • Organizer
      The 3^<rd> Austria/Japan Seminar on Comparative and Developmental Glycobiology
    • Place of Presentation
      埼玉、理研
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Yeast oligosaccharyltransferase generates free oligosaccharides during N-glycosylation2013

    • Author(s)
      Yoichiro Harada and Tadashi Suzuki
    • Organizer
      The Second Symposium RIKEN-Max Planck Joint Research Center for Systems Chemical Biology
    • Place of Presentation
      埼玉、理研
    • Related Report
      2013 Final Research Report
  • [Presentation] ドリコールオリゴ糖の品質管理機構の同定2013

    • Author(s)
      原田 陽一郎、中嶋 和紀、根岸 由紀、安形 高志、谷口 直之、鈴木 匡
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ドリコールオリゴ糖前駆体の品質管理機構2013

    • Author(s)
      原田 陽一郎、中嶋 和紀、根岸 由紀、安形 高志、谷口 直之、鈴木 匡
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪(大阪国際交流センター)
    • Related Report
      2013 Annual Research Report
  • [Presentation] The generation of free oligosaccharides in eukaryotes2013

    • Author(s)
      Yoichiro Harada
    • Organizer
      The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology
    • Place of Presentation
      埼玉(理化学研究所)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Yeast oligosaccharyltransferase generates free oligosaccharides during N-glycosylation2013

    • Author(s)
      Yoichiro Harada and Tadashi Suzuki
    • Organizer
      The Second Symposium RIKEN-Max Planck Joint Research Center for Systems Chemical Biology
    • Place of Presentation
      埼玉(理化学研究所)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 出芽酵母オリゴ糖転移酵素はN型糖鎖前駆体から糖鎖を遊離する活性を持つ2012

    • Author(s)
      原田 陽一郎、鈴木 匡
    • Organizer
      第31回 日本糖質学会
    • Place of Presentation
      鹿児島市民文化ホール
    • Related Report
      2012 Research-status Report
  • [Presentation] 酵母オリゴ糖転移酵素の加水分解活性の証明2012

    • Author(s)
      原田 陽一郎、鈴木 匡
    • Organizer
      第85回 日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Book] Handbook of Glycosyltransferases and Related Genes2014

    • Author(s)
      Taniguchi N, Honke K, Fukuda M, Narimatsu H, Yamaguchi Y, Angata T. (Eds.)
    • Publisher
      Springer (In pres)
    • Related Report
      2013 Final Research Report
  • [Book] Handbook of Glycosyltransferases and Related Genes2014

    • Author(s)
      Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., Angata, T. (Eds.)
    • Total Pages
      670
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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