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Study of the biologogical functions of the generation of free oligosaccharides

Research Project

Project/Area Number 24770136
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

HIRAYAMA Hiroto  独立行政法人理化学研究所, 糖鎖代謝学研究チーム, 協力研究員 (50525847)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords糖鎖代謝 / 遊離糖鎖 / 出芽酵母 / 遊離糖鎖解析 / 新規遺伝子探索
Research Abstract

In eukaryotes, free N-glycans (fNGs), derived from either N-glycans from glycoproteins or their dolichol-linked precursors, are accumulated in the cytosol [1,2]. To gain insight into the biological significance of fNGs in budding yeast (S. cerevisiae), the free glycans under various culture conditions were examined. We then serendipitously observed the accumulation of novel types of free glycans when cells were cultured with a media containing mannose as a sole carbon source. The generation of the novel free glycans was repressed by the presence of glucose in media. Furthermore, cells defect in one of transcription factor caused excess formation of the free glycans and show cell wall defect. These results clearly indicate that the generation of the novel free glycans is tightly regulated specifically with the transcription factor, and that excess generation of free glycans may lead to the compromised cell wall formation.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (22 results)

All 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (16 results) (of which Invited: 2 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Eukaryotic oligosaccharyltransferase generates free oligosaccharides during N-glycosylation2013

    • Author(s)
      Harada Y., Buser R., Ngwa EM., Hirayama H., Aebi M., Suzuki T.
    • Journal Title

      J Biol Chem

      Volume: 288(45) Pages: 32673-84

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ” Eukaryotic oligosaccharyltransferase generates free oligosaccharides during N-glycosylation.”2013

    • Author(s)
      Harada Y., Buser R., Ngwa EM., Hirayama H., Aebi M., Suzuki T.
    • Journal Title

      J Biol Chem.

      Volume: 288(45) Pages: 32673-32684

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 出芽酵母の生成するO-結合型糖鎖様構造を持つ遊離糖鎖とその機能について2013

    • Author(s)
      平山弘人、鈴木匡
    • Organizer
      第86回日本生化学会年会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] 出芽酵母の遊離糖鎖生成とその調節について2013

    • Author(s)
      平山弘人、鈴木匡
    • Organizer
      第45回酵母遺伝学フォーラム
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] 出芽酵母の生成するO-結合型糖鎖様構造を持つ遊離糖鎖とその機能について2013

    • Author(s)
      平山弘人、鈴木匡
    • Organizer
      第32回日本糖質学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] Discovery of the novel type of free glycans in Saccharomyces cerevisiae2013

    • Author(s)
      Hiroto Hirayama, Tadashi Suzuki
    • Organizer
      Japan-Austria joint seminar
    • Place of Presentation
      Wako, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Discovery of the novel type of free glycans in Saccharomyces cerevisiae2013

    • Author(s)
      Hiroto Hirayama, Tadashi Suzuki
    • Organizer
      RIKEN–Max Planck Joint Research Center for Systems Chemical Biology The 2nd Symposium
    • Place of Presentation
      Wako, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Discovery of the novel type of free glycans in Saccharomyces cerevisiae2013

    • Author(s)
      Hirayama H., Suzuki T.
    • Organizer
      Max Planck Joint Research Center for Systems Chemical Biology The 2nd Symposium
    • Place of Presentation
      埼玉
    • Related Report
      2013 Annual Research Report
  • [Presentation] Discovery of the novel type of free glycans in Saccharomyces cerevisiae2013

    • Author(s)
      Hirayama H., Suzuki T.
    • Organizer
      Japan-Austria joint seminar
    • Place of Presentation
      埼玉
    • Related Report
      2013 Annual Research Report
  • [Presentation] 出芽酵母の生成するO-結合型糖鎖様構造を持つ遊離糖鎖とその機能について2013

    • Author(s)
      平山弘人、鈴木匡
    • Organizer
      第32回 日本糖質学会年会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] 出芽酵母の生成するO-結合型糖鎖様構造を持つ遊離糖鎖とその機能について2013

    • Author(s)
      平山弘人、鈴木匡
    • Organizer
      第86回 日本生化学会年会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 出芽酵母の生成する遊離糖鎖とその機能について2013

    • Author(s)
      平山弘人 鈴木匡
    • Organizer
      第86回 日本生化学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 出芽酵母における遊離O-型糖鎖の生成およびその調節機構ついて2012

    • Author(s)
      平山弘人、鈴木匡
    • Organizer
      第85回日本製科学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 出芽酵母の持つ未知のエンド型O-マンノシダーゼによる遊離糖鎖の生成2012

    • Author(s)
      平山弘人、鈴木匡
    • Organizer
      第31回日本糖質学会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Final Research Report
  • [Presentation] 未知のエンド型O-マンノシダーゼによる遊離糖鎖の生成機構について2012

    • Author(s)
      平山弘人、鈴木匡
    • Organizer
      第44回酵母遺伝学フォーラム
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 未知のエンド型O-マンノシダーゼによる遊離糖鎖の生成機構について2012

    • Author(s)
      平山弘人
    • Organizer
      第44回 酵母遺伝学フォーラム
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] 出芽酵母の持つ未知のエンド型O-マンノシダーゼによる遊離糖鎖の生成2012

    • Author(s)
      平山弘人
    • Organizer
      第31回 日本糖質学会年会
    • Place of Presentation
      鹿児島
    • Related Report
      2012 Research-status Report
  • [Presentation] 出芽酵母における遊離O-型糖鎖の生成およびその調節機構ついて2012

    • Author(s)
      平山弘人
    • Organizer
      第85回 日本生化学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Book] Handbook of glycosyltransferases and related genes2013

    • Author(s)
      Hirayama H., Suzuki T.
    • Total Pages
      1707
    • Publisher
      Springer Japan
    • Related Report
      2013 Annual Research Report
  • [Book] GMPPA, B, Handbook of glycosyltransferases and related genes

    • Author(s)
      Hirayama H., Suzuki T.
    • Publisher
      Springer Japan
    • Related Report
      2013 Final Research Report
  • [Remarks] Hirayama H.,"Purification and Analysis of Free Oligosaccharides in Saccharomyces cerevisiae"Glycoscience Protocol Online Database

    • URL

      http://jcggdb.jp/GlycoPOD/

    • Related Report
      2013 Final Research Report
  • [Remarks] GlycoPOD

    • URL

      http://jcggdb.jp/GlycoPOD/

    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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