| Project/Area Number |
24770138
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
TOGAYACHI Akira 独立行政法人産業技術総合研究所, 糖鎖創薬技術研究センター, 主任研究員 (60392635)
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| Co-Investigator(Renkei-kenkyūsha) |
KAJI Hiroyuki 産業技術総合研究所, 糖鎖創薬技術研究センター・標的糖鎖探索チーム, 研究チーム長 (80214302)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糖鎖 / ポリラクトサミン / 糖転移酵素 / β1,3-N-アセチルグルコサミン転移酵素 / 糖タンパク質 / グライコプロテオミクス解析 / 免疫系 / β1,3- N-アセチルグルコサミン転移酵素 / 免疫 / ポリ-N-アセチルラクトサミン / N-アセチルグルコサミン転移酵素 / グライコプロテオミクス / 糖鎖機能 |
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| Outline of Final Research Achievements |
Glycosyltransferase gene-deficient mice show various abnormalities in biological functions. To analyze the mechanism, it is important to identify the glycoproteins targeted by the glycosyltransferases (glycan carrier molecules). In this study, we focused on polylactosamine glycans and identified the carrier molecules of polylactosaminoglycans using glycoproteomics technology. Glycopeptides were captured with the lectins specifically bind to polylactosamine glycans. Comparative glycoproteome analysis of the captured glycopeptides identified the target glycoprotein candidates. Bioinformatics analysis of the obtained carrier molecules found common characteristics and tendency in the molecular nature.
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