| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Outline of Final Research Achievements |
Intracellular temperature is crucial role for many cellular processes such as gene expression and cellular metabolism. In this study, we developed a new genetically encoded fluorescent temperature indicator for monitoring temperature changing in a single living cell by combination a temperature sensitive fluorescent protein and a temperature less sensitive protein. To monitor the intracellular organelle specific temperature dynamics, we succeeded in expression the temperature indicator in mitochondria, nucleus, and plasma membrane. And also, we successfully monitored heat production in mitochondria of single cell with chemical stimulation.
|
