Monitoring temperature inside a single cell with a novel genetically encoded fluorescent temperature indicator
| Project/Area Number |
24770174
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 温度 / 蛍光タンパク質 / 生物物理 / 細胞生物学 |
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| Outline of Final Research Achievements |
Intracellular temperature is crucial role for many cellular processes such as gene expression and cellular metabolism. In this study, we developed a new genetically encoded fluorescent temperature indicator for monitoring temperature changing in a single living cell by combination a temperature sensitive fluorescent protein and a temperature less sensitive protein. To monitor the intracellular organelle specific temperature dynamics, we succeeded in expression the temperature indicator in mitochondria, nucleus, and plasma membrane. And also, we successfully monitored heat production in mitochondria of single cell with chemical stimulation.
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Report
(4 results)
Research Products
(7 results)
