| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
In this study, I have tried to address the role of the nucleolus in cellular differentiation. I found that rRNA transcription was downregulated during hematopoietic differentiation in both human leukemia cell line and mouse hematopoietic stem cells. In response to the inhibition of rRNA transcription, nucleolar protein MYBBP1A was translocated from the nucleolus to the nucleoplasm and interacted with c-Myc, which is one of the master regulator of hematopoietic differentiation. MYBBP1A attenuated transcriptional activity of c-Myc and promoted megakaryocytic differentiation. I also found that hematopoietic differentiation was sufficiently induced by specific suppression of rRNA transcription using actinomycin D or siRNA for TIF-IA. These results suggest that the downregulation of rRNA transcription accelerates cellular differentiation at least in hematopoietic cells.
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