| Project/Area Number |
24770181
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
OKADA Maiko 聖マリアンナ医科大学, 医学(系)研究科(研究院), 助教 (00572330)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タンパク分解 / ユビキチン / 乳癌 / エストロゲン / 細胞周期 |
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| Research Abstract |
In this study, to reveal the novel mechanism of fat-soluble ligands regulating the proper protein expression, "Ligand-dependent protein degradation system" was investigated. Estrogen focused on as a ligand, the identification and functional analysis of the degradation substrates in an estrogen signaling was performed. As a result, the mitotic key regulator was degraded through Ubiquitin-proteasome system in an estrogen-dependent manner. moreover, it was shown that estrogen accelerated transition from mitosis to G1 phase. These results suggest that estrogen is related in the mitotic regulations through promoting the degradation of mitotic reugulator at M phase.
This study shows that estrogen regulates target proteins level through two pathway, the protein degradation related in M/G1 phase and well known gene expression pathway in G1/S phase, providing a new insight into the mechanism of the estrogen signaling.
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