| Project/Area Number |
24770218
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
YABE Taijiro 基礎生物学研究所, 分子発生学研究部門, 助教 (30470074)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ゼブラフィッシュ / 細胞分化 / 胚発生 / 尾部形成 / zebrafish |
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| Research Abstract |
In zebrafish development, tail paraxial mesoderm is sequentially generated from mesodermal progenitor cells locate at the surface of tailbud. We previously showed two transcriptional factors, spt and ntl, had important roles in these process. In this study we tried to identify the molecular mechanisms regulating expression of msgn1 during tail paraxial mesoderm differentiation.
First we identified msgn1 promoter region which is enough to recapture the endogenous msgn1 expression in zebrafish embryo. Reporter assay using cultured cells revealed this promoter activity was positively regulated by Wnt singaling and transcriptional facter, Ntl, which were activated at mesodermal progenitor cells, suggesting msgn1 expressin was repressed at the teilbud region by the unidentified molecular mechanism and derepression of such repression triggered the differentiation of tail paraxial mesoderm.
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