| Project/Area Number |
24780038
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Horticulture/Landscape architecture
|
|---|
| Research Institution | National Agriculture and Food Research Organization |
|---|
Principal Investigator |
YAGI Masafumi 独立行政法人農業・食品産業技術総合研究機構, 花き研究所花き研究領域, 主任研究員 (40391403)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | カーネーション / 萎凋細菌病 / QTL / DNAマーカー / BACライブラリー / 病害抵抗性 / 連鎖地図 |
|---|
| Research Abstract |
'Karen Rouge' is the carnation cultivar with the bacterial wilt resistance derived from Dianthus capitatus ssp. andrzejowskianus. In order to identify the responsible gene for the resistance, we constructed a BAC (bacterial artificial chromosome) library using 'Karen Rouge' genomic DNA, whose coverage was estimated to be about 12 genome equivalents. By using this library, we determined the 91,790 nucleotide sequence surrounding the marker STS-WG44 that is tightly linked to the resistance, and identified six candidate genes for the resistance.
|
