Analysis of the interaction between host and virus proteins in plant resistance to tobamovirus.
| Project/Area Number |
24780043
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant pathology
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| Research Institution | Iwate Biotechnology Research Center |
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Principal Investigator |
SEKINE Ken-Taro 公益財団法人岩手生物工学研究センター, 生命科学研究部, 主任研究員 (30574058)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 植物 / ウイルス / 抵抗性タンパク質 / 相互作用 / 構造生物学 / ウイルス認識機構 / タンパク質間相互作用 / gene for gene resistance / トバモウイルス |
|---|
| Research Abstract |
The allelic L proteins, L1, L2, L3, and L4, showed increasing binding capacity to different tobamovirus CPs, suggesting the correlation between the binding affinity and CP recognition by L proteins. In this study, we performed mutational analysis on xxLxLxx beta-sheet motifs in Leucine-rich repeat of L4 protein and CP responsible for the recognition. The binding affinity between L and CP (or the stability of the complex containing these proteins) is important for generating resistance signals.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Functional differentiation in the LRR domains of closely related plant virus resistance proteins that recognize common Avr proteins
Author(s)
Sekine, K.-T. , Tomita, R., Takeuchi, S., Atsumi, G., Saitoh, H., Mizumoto, H., Kiba, A., Yamaoka, N., Nishiguchi, M., Hikichi, Y., and Kobayashi, K.
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Journal Title
Molecular Plant-Microbe Interactions
Volume: 25
Pages: 1219-1229
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[Journal Article] Amino acids in Tobamovirus coat protein controlling pepper L^<la> gene-mediated resistance
Author(s)
Mizumoto, H., Nakamura, I., Shimomoto, Y., Sawada, H., Tomita, R., Sekine, K.-T., Kiba, A., Nishiguchi, M., Kobayashi, K., and Hikichi, Y.
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Journal Title
Molecular Plant Pathology
Volume: 13
Pages: 915-922
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[Presentation] The binding affinity to viral coat proteins determines the recognition specificity of allelic L tobamovirus resistance proteins2012
Author(s)
Sekine, K.-T., Tomita, R., Atsumi, G., Chen, H., Kaido, M., Yamaoka, N., Nishiguchi, M., Okuno, T., Kobayashi, K.
Organizer
XV International Congress of Molecular Plant-Microbe Interactions
Place of Presentation
京都,京都国際会議場
Year and Date
2012-07-29
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