Analysis of the interaction between host and virus proteins in plant resistance to tobamovirus.

• Project/Area Number: 24780043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant pathology
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: SEKINE Ken-Taro 公益財団法人岩手生物工学研究センター, 生命科学研究部, 主任研究員 (30574058)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 植物 / ウイルス / 抵抗性タンパク質 / 相互作用 / 構造生物学 / ウイルス認識機構 / タンパク質間相互作用 / gene for gene resistance / トバモウイルス

Research Abstract

The allelic L proteins, L1, L2, L3, and L4, showed increasing binding capacity to different tobamovirus CPs, suggesting the correlation between the binding affinity and CP recognition by L proteins. In this study, we performed mutational analysis on xxLxLxx beta-sheet motifs in Leucine-rich repeat of L4 protein and CP responsible for the recognition. The binding affinity between L and CP (or the stability of the complex containing these proteins) is important for generating resistance signals.

Research Products

• [Journal Article] Breakdown of plant virus resistance: can we predict and extend durability of virus resistance? (2014)
  • Author(s): Kobayashi, K., Sekine, K.-T., Nishiguchi, M.
  • Journal Title: Journal of General Plant Pathology Volume: 印刷中 Issue: 4 Pages: 327-336
  • DOI: 10.1007/s10327-014-0527-1
  • NAID: 130004779388
  • Peer Reviewed
• [Journal Article] Functional Differentiation in the Leucine-Rich Repeat Domains of Closely Related Plant Virus-Resistance Proteins That Recognize Common Avr Proteins (2012)
  • Author(s): 関根健太郎
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 25 Issue: 9 Pages: 1219-1229
  • DOI: 10.1094/mpmi-11-11-0289
  • Peer Reviewed
• [Journal Article] Functional differentiation in the LRR domains of closely related plant virus resistance proteins that recognize common Avr proteins
  • Author(s): Sekine, K.-T. , Tomita, R., Takeuchi, S., Atsumi, G., Saitoh, H., Mizumoto, H., Kiba, A., Yamaoka, N., Nishiguchi, M., Hikichi, Y., and Kobayashi, K.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 25 Pages: 1219-1229
• [Journal Article] Amino acids in Tobamovirus coat protein controlling pepper L^<la> gene-mediated resistance
  • Author(s): Mizumoto, H., Nakamura, I., Shimomoto, Y., Sawada, H., Tomita, R., Sekine, K.-T., Kiba, A., Nishiguchi, M., Kobayashi, K., and Hikichi, Y.
  • Journal Title: Molecular Plant Pathology Volume: 13 Pages: 915-922
• [Presentation] トパモウイルス粒子長梓化変異体のL抵抗性タンパク質による認識の回避 (2014)
  • Author(s): 関根健太郎・冨田麗子・厚見剛・小林括平
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌市,札幌コンベンションセンター
  • Year and Date: 2014-06-02
• [Presentation] トバモウイルス粒子長桿化変異体のL抵抗性タンパク質による認識の回避 (2014)
  • Author(s): 関根健太郎，冨田麗子，厚見剛，小林括平
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 北海道札幌市
• [Presentation] タンパク質相互作用を介した植物のウイルス認識機構 (2014)
  • Author(s): 関根健太郎，冨田麗子，厚見剛，小林括平
  • Organizer: 第３２回日本植物細胞分子生物学会シンポジウム「植物の相互作用を紐解く最前線」
  • Place of Presentation: 岩手県盛岡市
  • Invited
• [Presentation] Avr機能獲得変異体を用いたトパモウイルス抵抗性タンパク質による認識機構の解析 (2013)
  • Author(s): 関根健太郎・冨田麗子・厚見剛・小林括平
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜市,岐阜大学
  • Year and Date: 2013-03-27
• [Presentation] Avr機能獲得変異体を用いたトバモウイルス抵抗性タンパク質による認識機構の解析 (2013)
  • Author(s): 関根健太郎・冨田麗子・厚見剛・小林括平
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜市
• [Presentation] The binding affinity to viral coat proteins determines the recognition specificity of allelic L tobamovirus resistance proteins (2012)
  • Author(s): Sekine, K.-T., Tomita, R., Atsumi, G., Chen, H., Kaido, M., Yamaoka, N., Nishiguchi, M., Okuno, T., Kobayashi, K.
  • Organizer: XV International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都,京都国際会議場
  • Year and Date: 2012-07-29
• [Presentation] The binding affinity to viral coat proteins determines the recognition specificity of allelic L tobamovirus resistance proteins. (2012)
  • Author(s): Ken-Taro Sekine, Reiko Tomita, Go Atsumi, Hui Chen, Masanori Kaido, Naoto Yamaoka, Masamichi Nishiguchi, Tetsuro Okuno, Kappei Kobayashi
  • Organizer: XV International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: Kyoto, Japan
• [Remarks] 岩手生物工学研究センター 植物病態分子研究チームホームページ
  • URL: http://ppathol.ibrc.or.jp/
• [Remarks] 岩手生物工学研究センター園芸資源研究部植物病態分子研究チームのホームページ
  • URL: http://ppathol.ibrc.or.jp/