Characterization of bacterial second messengers involved in the interactions with plants

• Project/Area Number: 24780045
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant pathology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: TAKEUCHI Kasumi 独立行政法人農業生物資源研究所, 植物・微生物間相互作用研究ユニット, 主任研究員 (40370663)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: バイオコントロール細菌 / シグナル伝達系 / 植物保護 / 二次代謝産物

Outline of Final Research Achievements

Bacteria are metabolically versatile in response to the environments. But little is known about second messengers involved in this changing. In this study, we focused on biocontrol bacterium Pseudomonas protegens CHA0 (previously called Pseudomonas fluorescens CHA0) which suppresses plant diseases by producing extracellular enzymes and secondary metabolites with antibiotic activity in the rhizosphere. We have shown that ppGpp appears to be essential for sustaining epiphytic fitness and biocontrol activity in P. protegens CHA0.

Research Products

• [Journal Article] Rhizoxin analogs contribute to the biocontrol activity of a newly isolated Pseudomonas strain (2015)
  • Author(s): Kasumi Takeuchi, Naomi Noda, Yuichi Katayose, Yoshiyuki Mukai, Hisataka Numa, Kosumi Yamada, Nobutaka Someya
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 28 Issue: 3 Pages: 333-342
  • DOI: 10.1094/mpmi-09-14-0294-fi
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 拮抗細菌のGac/Rsmシグナル伝達系のオンとオフ (2015)
  • Author(s): 竹内香純
  • Journal Title: 日本植物病理学会報 Volume: 81
  • NAID: 130005087920
  • Acknowledgement Compliant
• [Journal Article] 植物根圏に生息するPseudomonas属細菌の抗菌性制御メカニズムとその利用 (2014)
  • Author(s): 竹内香純
  • Journal Title: バイオコントロール研究会 Volume: 13 Pages: 55-62
  • Acknowledgement Compliant
• [Journal Article] 拮抗細菌の二次代謝を制御する因子の探索 (2014)
  • Author(s): 竹内香純
  • Journal Title: 植物感染生理談話会論文集 Volume: 49 Pages: 105-112
  • Acknowledgement Compliant
• [Journal Article] 細菌が「のほほんモード」に戻るとき-拮抗細菌のシグナル伝達系のオンとオフ- (2014)
  • Author(s): 竹内香純
  • Journal Title: 植物細菌病談話会論文集 Volume: 26 Pages: 119-128
  • Acknowledgement Compliant
• [Journal Article] Lon protease negatively affects GacA protein stability and expression of the Gac/Rsm signal transduction pathway in Pseudomonas protegens (2014)
  • Author(s): Kasumi Takeuchi, Wataru Tsuchiya, Naomi Noda, Rintaro Suzuki, Toshimasa Yamazaki, Dieter Haas
  • Journal Title: Environmental Microbiology Volume: 未定 Issue: 8 Pages: 2538-2549
  • DOI: 10.1111/1462-2920.12394
  • Peer Reviewed
• [Journal Article] バイオコントロール細菌の抗菌性制御機構～細菌にしっかり働いてもらうためには (2013)
  • Author(s): 竹内香純
  • Journal Title: 化学と生物 Volume: 51 Pages: 78-80
  • NAID: 10031146882
• [Journal Article] ppGpp controlled by the Gac/Rsm regulatory pathway sustains biocontrol activity in Pseudomonas fluorecens CHA0 (2012)
  • Author(s): Kasumi Takeuchi, Kosumi Yamada, Dieter Haas
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 11 Issue: 11 Pages: 1440-1449
  • DOI: 10.1094/mpmi-02-12-0034-r
  • Peer Reviewed
• [Presentation] Pseudomonas sp. Os17の植物保護能力にはリゾキシン類縁体が寄与する (2015)
  • Author(s): 竹内香純, 野田なほみ, 片寄裕一, 向井喜之, 沼寿隆, 山田小須弥, 染谷信孝
  • Organizer: 平成27年度日本植物病理学会創立100周年記念大会
  • Place of Presentation: 明治大学駿河台キャンパス
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] Pseudomonas属細菌の調節型small RNAの発現制御と抗菌性二次代謝産物 (2015)
  • Author(s): 竹内香純
  • Organizer: 第88回日本細菌学会総会 ワークショップ
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Lon protease negatively affects GacA protein stability and expression of the Gac/Rsm signal transduction pathway in Pseudomonas protegens (2014)
  • Author(s): Takeuchi K, Tsuchiya W, Noda N, Suzuki R, Yamazaki T, Haas D
  • Organizer: XVI International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: ギリシャ Rodos Palace Hotel
  • Year and Date: 2014-07-06 – 2014-07-10
• [Presentation] LonプロテアーゼによるGacAタンパク質の不安定化は環境ストレス依存的に起きる (2014)
  • Author(s): 竹内香純
  • Organizer: 第87回 日本細菌学会総会
  • Place of Presentation: タワーホール船堀
• [Presentation] バイオコントロール細菌の抗菌性制御のメカニズム (2014)
  • Author(s): 竹内香純
  • Organizer: 平成25年度 近畿中国四国農業試験研究推進会議 病害虫推進部会 問題別研究会
  • Place of Presentation: 福山市生涯学習プラザ
  • Invited
• [Presentation] Lon protease negatively regulates the Gac/Rsm signal transduction pathway in Pseudomonas protegens CHA0 (2013)
  • Author(s): Kasumi Takeuchi, Wataru Tsuchiya, Naomi Noda, Rintaro Suzuki, Toshimasa Yamazaki, Dieter Haas
  • Organizer: 14th International Conference on Pseudomonas
  • Place of Presentation: ローザンヌ大学（スイス）
• [Presentation] Small RNA-dependent regulatory mechanisms of antibiotic production in Pseudomonas fluorescens (2013)
  • Author(s): 竹内香純
  • Organizer: 第86回 日本細菌学会総会
  • Place of Presentation: 幕張メッセ
• [Presentation] ppGpp controlled by the Gac/Rsm regulatory pathway sustains biocontrol activity in Pseudomonas fluorescens CHA0 (2012)
  • Author(s): Kasumi Takeuchi, Kosumi Yamada, Dieter Haas
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都国際会議場
• [Remarks] （独）農業生物資源研究所 植物科学研究領域 植物・微生物間相互作用研究ユニット
  • URL: http://www.nias.affrc.go.jp/org/DivPlant/Plant-Microbe/
• [Remarks] （独）農業生物資源研究所 植物科学研究領域 植物・微生物間相互作用研究ユニット
  • URL: http://www.nias.affrc.go.jp/org/DivPlant/Plant-Microbe/