Function and regulation of D-amino acid in eukaryote

• Project/Area Number: 24780098
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: ITO Tomokazu 名古屋大学, 生命農学研究科, 助教 (90584970)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: D-アミノ酸 / D-セリン / D-アスパラギン酸 / D-セリンデヒドラターゼ / D-セリンデヒドラーゼ / ピリドキサールリン酸

Outline of Final Research Achievements

We developed a simple and rapid D-serine enzymatic assay kit applicable to determine D-serine content in human urine. We studied the reaction mechanisms of two D-serine metabolic enzymes, serine racemase and Zn2+-dependent D-serine dehydratase. We characterized the enzymatic propeｒties of putative D-aspartate biosynthetic enzyme in mammals, Got1l1, and analyzed the effects of knock-out of the Got1l1 on the D-aspartate production. The obtained results showed that the Got1l1 is not the major D-aspartate synthetic enzyme in mammals.

Research Products

• [Journal Article] PEGylated D-Serine Dehydratase as a D-Serine Reucing Agent (2015)
  • Author(s): Ito T., Takada H., Isobe K., Suzuki M., Kitaura Y., Hemmi H., Matsuda T., Sasabe J., Yoshimura T.
  • Journal Title: Journal of Parmacetuical and Biomedical Analysis Volume: in press Pages: 34-39
  • DOI: 10.1016/j.jpba.2014.12.044
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of the aminotransferase domain in Bacillus subtilis GabR, a pyridoxal 5'-phosphate-dependent transcriptional regulator (2015)
  • Author(s): Okuda K., Kato S., Ito T., Shiraki S., Kawase Y., Goto M., Kawashima S., Hemmi H., Fukada H., Yoshimura T
  • Journal Title: Molecular Microbiology Volume: 95 Issue: 2 Pages: 245-257
  • DOI: 10.1111/mmi.12861
  • Peer Reviewed
• [Journal Article] Is D-aspartate produced by glutamic-oxaloacetic transaminase -1 like 1(Got1l1), a putative aspartate racemase? (2015)
  • Author(s): Tanaka-Hayashi A, Hayashi S, Inoue R, Ito T, Konno K, Yoshida T, Watanabe M, Yoshimura T, Mori H
  • Journal Title: Amino Acids Volume: 47 Issue: 1 Pages: 79-86
  • DOI: 10.1007/s00726-014-1847-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Reaction mechanism of Zn2+-dependent D-serine dehydratase: role of a conserved tyrosine residue interacting with pyridine ring nitrogen of pyridoxal 5'-phosphate (2014)
  • Author(s): Ito T., Matsuoka M., Koga K., Hemmi H., Yoshimura T.
  • Journal Title: Journal of Biochemistry Volume: 156 Issue: 3 Pages: 173-180
  • DOI: 10.1093/jb/mvu035
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] D－アミノ酸代謝関連酵素ー構造・機能研究の最前線ー「D－セリンデヒドラターゼ」 (2014)
  • Author(s): 伊藤智和
  • Journal Title: BIOINDUSTRY Volume: 3 Pages: 24-32
• [Journal Article] Conserved pyridoxal protein that regulates Ile and Val metabolism. (2013)
  • Author(s): Ito T, Iimori J, Takayama S, Moriyama A, Yamauchi A, Hemmi H, Yoshimura T.
  • Journal Title: J Bacteriol. Volume: 195 Issue: 24 Pages: 5439-5449
  • DOI: 10.1128/jb.00593-13
  • Peer Reviewed
• [Journal Article] Catalyticmechanism of serine racemase fromDictyostelium discoideum. (2013)
  • Author(s): Ito T, Maekawa M, Hayashi S, Goto M,Hemmi H, Yoshimura T
  • Journal Title: Amino Acids Volume: 44 Issue: 3 Pages: 1073-1084
  • DOI: 10.1007/s00726-012-1442-4
  • Peer Reviewed
• [Journal Article] Metal ion dependency of serine racemase from Dictyostelium discoideum (2012)
  • Author(s): Ito T, Murase H, Maekawa M, Goto M, Hayashi S, Saito H, Maki M, Hemmi H, Yoshimura T
  • Journal Title: Amino Acids Volume: (印刷中) Issue: 4 Pages: 1567-1576
  • DOI: 10.1007/s00726-012-1232-z
  • Peer Reviewed
• [Presentation] Brevibacillus brevisの新奇ピリドキサール5’-リン酸(PLP)結合性転写調節因子の解析 (2014)
  • Author(s): 伊藤智和、竹中崇、邊見久、吉村徹
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2015-10-18
• [Presentation] D-SERINE METABOLISM IN CELLULAR SLIME MOLD AND EFFECTS OF D-SERINE DEHYDRATASE MUTATION (2014)
  • Author(s): Tomokazu ITO, Natsuki HAMAUCHI, Hisashi HEMMI, Tamao SAITO, Tohru YOSHIMURA
  • Organizer: 4th International Conference on Cofactors (ICC-04)
  • Place of Presentation: 宇都宮市
  • Year and Date: 2014-09-01
• [Presentation] Catalytic mechanism of PLP- and Zn2+-dependent D-serine dehydratase (2014)
  • Author(s): Tomokazu Ito, Mai Matsuoka, Hisashi Hemmi, and Tohru Yoshimura
  • Organizer: 4th International Conference on Cofactors (ICC-04)
  • Place of Presentation: Parma、Italy
  • Year and Date: 2014-08-25 – 2014-08-28
• [Presentation] 亜鉛依存性D-セリンデヒドラターゼの反応機構：PLPピリジン環窒素の相互作用残基(Y203)の変異体解析 (2014)
  • Author(s): 伊藤智和・松岡舞・邊見久・吉村徹
  • Organizer: 日本ビタミン学会第 65回大会
  • Place of Presentation: 姫路
  • Year and Date: 2014-06-13 – 2014-06-14
• [Presentation] 細胞性粘菌におけるD-セリン代謝酵素欠損の影響 (2014)
  • Author(s): 伊藤智和、濱内菜月、邊見久、吉村徹
  • Organizer: 2014年度日本農芸化学会大会
  • Place of Presentation: 明治大学
• [Presentation] イソロイシン、バリン代謝系に関与する新奇ビタミンB6タンパク質 (2013)
  • Author(s): 伊藤智和、飯盛淳平、邊見久、吉村徹
  • Organizer: 日本ビタミン学会第65回大会
  • Place of Presentation: 一橋大学・一橋講堂
• [Presentation] 真核微生物におけるD-セリン：代謝酵素と生理的意義 (2013)
  • Author(s): 伊藤智和
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] ピリドキサル5’-リンに依存するBacillus subtilis の転写制御因子GabR (2013)
  • Author(s): 奥田啓太、深田はるみ、伊藤智和、後藤 勝、吉村 徹
  • Organizer: 日本生物高分子学会2013年度大会
  • Place of Presentation: 学校法人常翔学園教育施設「大阪センター」
• [Presentation] 真核生物型D-セリンデヒドラターゼの反応機構：PLPピリジン環窒素の相互作用残基Y203の役割 (2013)
  • Author(s): 松岡舞、伊藤智和、邊見久、吉村徹
  • Organizer: 酵素補酵素研究会
  • Place of Presentation: 立命館大学
• [Presentation] D-アスパラギン酸合成酵素とされる哺乳動物Got1l1の酵素活性 (2013)
  • Author(s): 林修平、伊藤智和、邊見久、田中亜由美、吉田知之、森寿、吉村徹
  • Organizer: D-アミノ酸学会
  • Place of Presentation: 関西大学
• [Presentation] 亜鉛依存性D-セリンデヒドラターゼの反応機構：PLPピリジン環窒素の相互作用残基Y203の変異体解析 (2013)
  • Author(s): 松岡舞、伊藤智和、古賀和志、邊見久、吉村徹
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 細胞性粘菌におけるD-セリンデヒドラターゼ欠損の影響 (2013)
  • Author(s): 濱内菜月、伊藤智和、邊見久、齊藤玉緒、吉村徹
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 細胞性粘菌におけるD-セリンの代謝と機能 (2012)
  • Author(s): 伊藤智和、萩 泰典、濱内菜月、邊見 久、齊藤玉緒、吉村 徹
  • Organizer: 第8回D-アミノ酸研究会学術講演会
  • Place of Presentation: 滋賀医科大学
• [Presentation] 亜鉛に依存するD-セリンデヒドラターゼ：亜鉛の役割と反応機構の解析 (2012)
  • Author(s): 伊藤智和、松岡舞、邊見久、吉村徹
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 真核生物型D-セリンデヒドラターゼの触媒機構 (2012)
  • Author(s): 伊藤智和
  • Organizer: 2012年度酵素補酵素研究会
  • Place of Presentation: 名古屋大学