Function and regulation of D-amino acid in eukaryote
Project/Area Number |
24780098
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
|
Research Institution | Nagoya University |
Principal Investigator |
ITO Tomokazu 名古屋大学, 生命農学研究科, 助教 (90584970)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | D-アミノ酸 / D-セリン / D-アスパラギン酸 / D-セリンデヒドラターゼ / D-セリンデヒドラーゼ / ピリドキサールリン酸 |
Outline of Final Research Achievements |
We developed a simple and rapid D-serine enzymatic assay kit applicable to determine D-serine content in human urine. We studied the reaction mechanisms of two D-serine metabolic enzymes, serine racemase and Zn2+-dependent D-serine dehydratase. We characterized the enzymatic properties of putative D-aspartate biosynthetic enzyme in mammals, Got1l1, and analyzed the effects of knock-out of the Got1l1 on the D-aspartate production. The obtained results showed that the Got1l1 is not the major D-aspartate synthetic enzyme in mammals.
|
Report
(4 results)
Research Products
(23 results)
-
-
[Journal Article] Role of the aminotransferase domain in Bacillus subtilis GabR, a pyridoxal 5'-phosphate-dependent transcriptional regulator2015
Author(s)
Okuda K., Kato S., Ito T., Shiraki S., Kawase Y., Goto M., Kawashima S., Hemmi H., Fukada H., Yoshimura T
-
Journal Title
Molecular Microbiology
Volume: 95
Issue: 2
Pages: 245-257
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-