| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Some enzymes related to synthesis of beta-1,3-glucan were investigated in this study. The catalytic domain protein of beta-1,3-glucan synthase from Schizophyllum commune showed significant enzyme activity with UDPglucose. Glucosyltransferases were found in a cell wall autolysate and a culture medium of the fungal mycelia. In addition, novel bacterial beta-1,3-glucan phosphorylase and laminaridextrin phosphorylase were discovered and used for synthesis of beta-1,3-glucan and laminarioligosaccharides. Finally, water-soluble branched polysaccharide was synthesized in vitro using beta-1,3-glucan phosphorylase and bacterial glucosyltransferase.
|
