Characterization and application of beta-glucan related enzymes
Project/Area Number |
24780099
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
|
Research Institution | Mie University |
Principal Investigator |
ISONO Naoto 三重大学, 生物資源学研究科, 准教授 (70378321)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | β-1,3-グルカン / ラミナリオリゴ糖 / 糖転移酵素 / スエヒロタケ / ホスホリラーゼ / β-グルカン / シゾフィラン |
Outline of Final Research Achievements |
Some enzymes related to synthesis of beta-1,3-glucan were investigated in this study. The catalytic domain protein of beta-1,3-glucan synthase from Schizophyllum commune showed significant enzyme activity with UDPglucose. Glucosyltransferases were found in a cell wall autolysate and a culture medium of the fungal mycelia. In addition, novel bacterial beta-1,3-glucan phosphorylase and laminaridextrin phosphorylase were discovered and used for synthesis of beta-1,3-glucan and laminarioligosaccharides. Finally, water-soluble branched polysaccharide was synthesized in vitro using beta-1,3-glucan phosphorylase and bacterial glucosyltransferase.
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Report
(4 results)
Research Products
(14 results)