Development of the vaccine delivery method against fish diseases using virus-like particle

Research Project

Project/Area Number	24780198
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General fisheries
Research Institution	Fisheries Research Agency
Principal Investigator	TAKANO Tomokazu 独立行政法人水産総合研究センター, 増養殖研究所, 研究員 (40533998)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	ウイルス様粒子 / ワクチン / ベータノダウイルス / ウイルス性神経壊死症ウイルス
Research Abstract	Virus-like particles (VLP) may be utilized as vaccine vector of oral and/or immersion vaccination of fish. Hence, generation of VLP from the recombinant coat protein of viral nervous necrosis (VNN) was assessed in this study. Recombinant coat protein was generated by Escherichia coli BL21 harboring the coat protein gene. The recombinant coat protein was effectively purified by a sucrose density-gradient centrifugation method. However, irregular-shaped VLPs were observed in the sample by electron microscopy.

Report

(3 results)