Analysis of the denitrifying bacterial community in a biofilter packing media

Research Project

Project/Area Number	24780263
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Zootechnical science/Grassland science
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	YASUDA Tomoko 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所・畜産環境研究領域, 主任研究員 (50391371)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	畜産環境 / 生物脱臭
Outline of Final Research Achievements	The purpose of this study is to understand microbial properties, especially regarding denitrification, of a biofilter packing media. An in situ hybridization followed by a scanning electron microscopic observation was attempted to apply to biofilter media in order to obtain both genetic and morphological information. However, it was not possible to distinguish target cells from nontarget ones and needed more challenging. Microbial community structures of the biofilter packing media were also investigated. The results revealed the differences in microbial community structures between pumice tuff and rockwool mixture. It could be hypothesized that the proportion of bacteria possessing denitrification activity increased in pumic tuff.