| Project/Area Number |
24780290
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | National Institute of Infectious Diseases (2013)
Tokyo University of Science (2012) |
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Principal Investigator |
KAWAI yasuhiro 国立感染症研究所, 動物管理室, 研究員 (00416281)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | pKnox1 / 精原細胞 / 増殖 |
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| Research Abstract |
The maintenance, self-renewal, and differentiation of spermatogonial stem cells (SSCs) are tightly regulated processes that are critical for normal spermatogenesis. TALE family homeodomain transcription factors, including pKnox1, are demonstrated to be essential for hematopoietic stem cell maintenance and self-renewal. pKnox1 is preferentially expressed in the male germ cell lineage, there is a possibility that pKnox1 is involved in SSC maintenance and differentiation. Our study shown that pKnox1 deleted spermatogonia was arrested at c-kit positive spermatogonia and proliferative capacity of c-kit positive spermatogonia was significantly reduced. Moreover, at 1 week after pKnox1 deletion induced by tamoxifen treatment, spermatogenesis was normal, although proliferation related genes expression pattern were dynamically changed compared with control testes. These results indicated that pKnox1 is required for proper proliferation of c-kit positive spermatogonia.
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