| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
Denitrification is a microbial respiratory process converting nitrate to dinitrogen gas. This process contributes to the emission of N2O, an important greenhouse gas. Denitrifiers responsible for production of N2O in crop residue are largely unknown. The objectives of this research were to find a 'hot spot' of N2O emissions on crop (cabbage) residues and to isolate the active denitrifiers from the 'hot spot'. A new apparatus was designed to sample the N2O gas from the surface of the cabbage residue at small scales without destruction of the residue. The N2O emission from the surface of cabbage residue in the field was evaluated using the sampling apparatus. As a result, large amounts of emissions were observed from rotten parts of the residue. Thereafter, denitrifires were recovered from the rotten parts of residue where the highest N2O emission was observed. Sixty denitrifiers were isolated and assigned to five genera.
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