Evaluation of gene delivery mechanism based on quantitative visualization of cellular defense system against exogenous genes for high-efficiency gene therapy
| Project/Area Number |
24790036
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | National Institute of Advanced Industrial Science and Technology (2014)
Institute of Physical and Chemical Research (2013)
Hokkaido University (2012) |
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Principal Investigator |
SASAKI Akira 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (30580162)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 遺伝子デリバリー / 蛍光イメージング / 1分子計測 / 画像相関 / 酵素活性 / 画像相関分光法 / DNA分解酵素 / 蛍光相互相関分光法 / 生物物理 / DNA / １分子計測 |
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| Outline of Final Research Achievements |
The Purpose of this study is to visualize intracellular diffusion and degradation of exogenous genes quantitatively. We have newly developed raster image cross-correlation spectroscopy (ccRICS) setup. Degradation activity of DNA in living cells was successfully monitored as a movie through a direct measurement by the developed ccRICS system. Our result shows that the DNA degradation activity in living cells differs between cell lines. The achievement reveals a defense machinery against exogenous DNA invasion and determine its activity. The work will contribute a development of nucleic acid medicine and gene therapy.
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Report
(4 results)
Research Products
(10 results)
