Development of siRNA based anti Nidovirus drug against mosquito

• Project/Area Number: 24790134
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental pharmacy
• Research Institution: Fukuoka University
• Principal Investigator: SATHO Tomomitsu 福岡大学, 薬学部, 助教 (90369025)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: コガタアカイエカ / ニドウイルス / メソニウイルス / シマカ

Research Abstract

Dak Nong Virsu (DKNV) was isolated from Culex tritaeniorhynchus collected in Vietnam. Electron microscopic analysis showed that DKNV is enveloped, spherical virus with an diameter of 80nm. The complete genome sequence of DKNV was 20256bp and its genome appeared to contain at least seven ORFs. DKNV RdRp sequence was most similar to that of the members of the species Alphamesonivirus1 (Cavally virus and Nam Dinh virus) in the genus Alphamesonivirus of the family Mesoniviridase.

Research Products

• [Journal Article] Characterization of Dak Nong virus, an insect nidovirus isolated from Culex mosquitoes in Vietnam (2013)
  • Author(s): Ryusei Kuwata, Tomomitsu Satho, Haruhiko Isawa, Nguyen Thi Yen, Tran Vu Phong, Phan Thi Nga, Tomokazu Kurashige, Yukihiro Hiramatsu, Yuki Fukumitsu, Keita Hoshino, Toshinori Sasaki, Mutsuo Kobayashi, Tetsuya Mizutani, and Kyoko Sawabe
  • Journal Title: Arch Virol Volume: 158(11) Pages: 2273-84
• [Journal Article] Dak Nong virus, a novel insect nidovirus isolated from Culex mosquitoes in Vietnam (2013)
  • Author(s): Kuwata R., Satoh T., Isawa H., Yen N.T., Phong T.V., Nga P.T., Kurashige T., Hiramatsu Y., Fukumitsu Y., Hoshino K., Sasaki T., Kobayashi M., Mizutani T., Sawabe K
  • Journal Title: Arch. Virol Volume: 158(in press) Issue: 11 Pages: 2273-84
  • DOI: 10.1007/s00705-013-1741-4
  • Peer Reviewed
• [Journal Article] Temporal and Spatial Distribution of Dengue Vector Mosquitoes and their Habitat Patterns in Penang Island, Malaysia (2013)
  • Author(s): Rahman G. M. Saifur, Ahmad Abu Hassan, Hamady Dieng, Md Rawi Che Salmah, Ahmad Ramli Saad, and Tomomitsu Satho
  • Journal Title: Journal of the American Mosquito Control Association Volume: 29(1) Pages: 33-43
  • Peer Reviewed
• [Journal Article] Update on temporal and spatial abundance of dengue vectors in Penang, Malaysia. (2012)
  • Author(s): Rahman G. M. Saifur, Abu Hassan Ahmad, Hamady Dieng, Hamdan Ahmad, Mohd Ravi Che Salmah, Tomomitsu Satho, Ahmad Ramli Saad, Ronald Enrique Morales
  • Journal Title: Journal of the American Mosquito Control Association Volume: 28(2) Pages: 84-92
  • Peer Reviewed
• [Journal Article] Elevation of dopamine level reduces host-seeking activity in the adult female mosquito Aedes albopictus (2012)
  • Author(s): Fukumitsu Y., Irie K., Satho T., Aonuma H., Dieng H., Ahmad A.H., Nakashima Y., Mishima K., Kashige N. and Miake F
  • Journal Title: Parasite Vector Volume: 5(92) Issue: 1 Pages: 92-92
  • DOI: 10.1186/1756-3305-5-92
  • Peer Reviewed
• [Presentation] ベトナム捕集蚊より分離された新規ニドウイルスの性状解析 (2013)
  • Author(s): 鍬田龍星佐藤朝光伊澤晴彦Yen, Nguyen Thi Phong, Tran Vu Nga, Phan Thi星野啓太佐々木年則水谷哲也前田健小林睦生沢辺京子
  • Organizer: 第61回日本ウイルス学会学術集会
  • Year and Date: 2013-11-10
• [Presentation] ベトナム捕集蚊より分離された新規ニドウイルスの性状解析 (2013)
  • Author(s): 鍬田龍星 佐藤朝光 伊澤晴彦 Yen, Nguyen Thi Phong, Tran Vu Nga, Phan Thi 星野啓太 佐々木年則 水谷哲也 前田健 小林睦生 沢辺京子
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] A decrease of host-seeking behavior in virgin female appears without elevation of dopamine levels in the adult mosquito Aede albopictus (2012)
  • Author(s): 福光 由起、入江 圭一、佐藤 朝光、中島 幸彦、三島 健一、鹿志毛 信広、見明 史雄
  • Organizer: Joint International Tropical Medicine Meeting 2012
  • Place of Presentation: タイ・バンコク
• [Remarks] 福岡大学研究者情報