Effects of immune-complex formation on the biological activities of anti-TNFalpha monoclonal antibody products

Research Project

Project/Area Number	24790183
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Medical pharmacy
Research Institution	National Institute of Health Sciences
Principal Investigator	TADA Minoru 国立医薬品食品衛生研究所, 生物薬品部, 室長 (50506954)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	抗体医薬品 / TNFα
Outline of Final Research Achievements	To reveal the differences in the biological activities of anti-TNFα monoclonal antibody products, effects of immune-complex formation on their biological activities were examined. The molecular sizes of immune-complex formed by TNFα and anti-TNFα monoclonal antibodies were different in each antibody. FcγR reporter cell assay showed that the activations of FcγR by each antibody were dependent on the size of immune-complex. These results suggest that the difference in the formation of immune-complex is an important property characterizing anti-TNFα monoclonal antibody products.