Function of a helicase-related factor during mouse neural crest development

• Project/Area Number: 24790189
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Tohoku University
• Principal Investigator: WATANABE Yusuke 東北大学, 加齢医学研究所, 助教 (20562333)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 神経堤 / 発生 / マウス / 神経堤細胞

Research Abstract

Sno1 is expressed in neural crest cells of mouse embryo, and Sno1 conditional KO embryos in neural crest lineage showed increased cell death and hypoplastic craniofacial structure. I found that Sno1 interacts with a Histone binding protein. Sno1 can activate gene transcription, and Sno1-C terminal region accumulates at the site of DNA-double strand breaks. Taken together, Sno1 might be involved in gene transcription and DNA-damage repair in developing neural crest cells.

Research Products

• [Journal Article] Gain-of-Function Mutations in RIT1 Cause Noonan Syndrome, a RAS/MAPK Pathway Syndrome (2013)
  • Author(s): Aoki Y, Niihori T, Banio T, Okamoto N, Mizuno S, Kurosawa K, Ogata T, Takada F, Yano M, Ando T, Hoshika T, Barnett C, Ohashi H, Kawame H, Hasegawa T, Okutani T, Nagashima T, Hasegawa S, Funayama R, Nagashima T Nakayama K, Inoue S, Watanabe Y, Oeura T, Matsubara Y
  • Journal Title: The American Journal of Human Genetics Volume: Volume 93, Issue 1 Issue: 1 Pages: 173-180
  • DOI: 10.1016/j.ajhg.2013.05.021
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 10 gene regulation in the second heart field by Tbx1, Nkx2-5, and Islet1 reveals a genetic switch for down-regulation in the myocardium. (2012)
  • Author(s): Watanabe Y.
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Issue: 45 Pages: 18273-18280
  • DOI: 10.1073/pnas.1215360109
  • Peer Reviewed
• [Presentation] Strawberry Notch1, a helicase-related chromatin factor, regulates development of mouse preimplantation development (2013)
  • Author(s): Yusuke Watanabe
  • Organizer: 第8回研究所ネットワークシンポジウム
  • Place of Presentation: 京都大学医学部創立百周年記念施設「芝蘭会館」
• [Presentation] Strawberry Notch 1, a helicase-related factor, is required for development of mouse preimplantation embryo (2013)
  • Author(s): Yusuke Watanabe, Yoshikazu Hirate, Hiroshi Sasaki, Toshihiko Ogura
  • Organizer: The 61st NIBB Conference, Cellular Community in Mammalian Embryogenesis
  • Place of Presentation: Okazaki Conference Center
• [Presentation] Strawberry notch1, a helicase-related chromatin factor, regulates development of mouse preimplantation embryo. (2013)
  • Author(s): Yusuke Watanabe
  • Organizer: 第８回研究所ネットワーク国際シンポジウム
  • Place of Presentation: 京都大学医学部創立百周年記念施設「芝蘭会館」
• [Presentation] Strawberry Notch1, a Helicase-Related Factor, Is Required for Development of Mouse Preimplantation Embryo (2013)
  • Author(s): Yusuke Watanabe, Yoshikazu Hirate, Hiroshi Sasaki, Toshihiko Ogura
  • Organizer: The 61st NIBB Conference, Cellular Community in Mammalian Embryogenesis
  • Place of Presentation: Okazaki Conference Center, Aichi
• [Presentation] Nkx2-5, Islet1, Tbx1による二次心臓形成領域でのFgf10の発現制御機構 (2012)
  • Author(s): 渡邉裕介
  • Organizer: 第11回心血管発生研究会
  • Place of Presentation: 福島県郡山市
  • Year and Date: 2012-10-19
• [Presentation] Strawberry Notch1 involves in mammalian preimplantation development (2012)
  • Author(s): Yusuke Watanabe, Minoru Omi, Toshihiko Ogura
  • Organizer: 第35回分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Strawberry Notch1 is required for mouse preimplantation development (2012)
  • Author(s): Yusuke Watanabe, Minoru Omi, Toshihiko Ogura
  • Organizer: 第45回発生・第64回細胞生物学会合同年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Strawberry Notch1 is required for mouse preimplantation development (2012)
  • Author(s): Yusuke Watanabe, Minoru Omi, Toshihiko Ogura
  • Organizer: 第４５回発生・第６４回細胞生物学会合同年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Strawberry Notch1 involves in mammalian preimplantation development (2012)
  • Author(s): Yusuke Watanabe, Minoru Omi, Toshihiko Ogura
  • Organizer: 第３５回分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Nkx2-5, Islet1, Tbx1による二次心臓形成領域でのFgf10の発現制御機構
  • Author(s): 渡邉裕介
  • Organizer: 第１１回心血管発生研究会
  • Place of Presentation: 福島県郡山市