| Project/Area Number |
24790216
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | National Center of Neurology and Psychiatry (2013)
Okayama University (2012) |
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Principal Investigator |
SATO AKIRA 独立行政法人国立がん研究センター, 研究所, 研究員 (40530663)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 細胞死制御 / ネクローシス / アポトーシス / ATF3 / Lamin-B1 / Cytokeratin-19 / MicroRNA / siRNA / Hsp90 / 転写因子 / マイクロアレイ / 5-Fluoro-2'-deoxyuridine |
|---|
| Research Abstract |
Using mouse tumor cells in culture, we have been studying mechanisms of cell-death, necrosis and apoptosis, caused by an anti-cancer drug. A cellular microRNA (miRNA) was found to regulate the two death-modes. This miRNA is up-regulated in apoptosis-fated cells but not in necrosis-fated cells. We introduced to the necrosis-fated cells an over-expression of the miRNA by transfecting an miRNA-mimic, and found that the cells then underwent apoptosis. We then found that the cells now changed to die by apoptosis show a decrease in the nuclear scaffold protein lamin-B1.
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