Investigation on the sex difference in the epigenetic regulation of the CRF gene in the hypothalamus
| Project/Area Number |
24790241
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Nippon Medical School |
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Principal Investigator |
HIGO Shimpei 日本医科大学, 医学部, 助教 (50623922)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ストレス / 性差 / CRF / stress / epigenomic / sex difference / epigenomics |
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| Research Abstract |
Male and female respond differently to various stress. In this study, the epigenetic status of the CRF gene promoter in the praventricular nucleus (PVN) of the hypothalamus of male and female rats were investigated in order to elucidate the underlying mechanisms of the sex differences in the stress response. No sex difference was observed in basal CRF expression in the PVN, whereas the restraint-stress induced increase in the CRF expresson in the female rats were larger than that of the male. Regarding the epigenetic status of the CRF promoter region in the PVN, no sexdifference was observed in the DNA methylation profile, whereas the level of histone acetylation after restraint stress tends to higher in the female. These results indicate the involvement of the histone modification but not DNA methylation in sexdifference in the CRF expression.
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Report
(3 results)
Research Products
(16 results)
