The mechanism of development of the suprachiasmatic nucleus

• Project/Area Number: 24790288
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kumamoto University
• Principal Investigator: HATAKEYAMA Jun 熊本大学, 発生医学研究所, 助教 (90404350)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 視交叉上核 / 神経発生 / 核形成 / Dbx1 / 核構造 / 発生 / 国際研究者交流（フランス） / 国際研究者交流フランス / 神経幹細胞

Research Abstract

Every kingdom of life-bacteria, plants, and animal- has 24-hours rhythms. In mammal, the suprachaismatic nucleus (SCN) works as a center of the biological clock and the function is well known. However the mechanism of development of that is poorly understood. I found that homeobox genes, Lhx1 and Dbx1, are expressed by differentiating neurons and neural progenitors of SCN respectively. And Ror-alpha is also expressed by differentiating neurons of SCN. In Dbx1 knockout mice, neurons of SCN which are Ror-alpha positive cells were decreased. This result indicated that Dbx1 is important for the formation of SCN.

Research Products

• [Journal Article] Electroporation for the chick embryo CNS (2014)
  • Author(s): Hatakeyama J, and Shimamura K
  • Journal Title: Electroporation Methods and Neuroscience Springer Volume: (in press)
  • Peer Reviewed
• [Journal Article] Reconstruction of a seminiferous tubule-like structure in a 3 dimensional culture system of re-aggregated mouse neonatal testicular cells within a collagen matrix (2014)
  • Author(s): Zhang J, Hatakeyama J, Eto K, and Abe S-I
  • Journal Title: General and Comparative Endocrinology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates (2014)
  • Author(s): Hatakeyama J, Wakamatsu Y, Nagafuchi A, Kageyama R, and Shimamura K
  • Journal Title: (Development内の4月最も読まれたTop20に選出, Faculty of 1000に選出) Volume: 141 Pages: 1671-1682
  • Peer Reviewed
• [Journal Article] The adherens junction in the apical endfoot serves as a central site for active Notch signaling to control neurogenesis in vertebrates (2014)
  • Author(s): Hatakeyama, J., Wakamatsu, Y., Nagafuchi, A., Kageyama, R., Shigemoto, R., and Shimamura, K.
  • Journal Title: Development Volume: 141 Issue: 8 Pages: 1671-1682
  • DOI: 10.1242/dev.102988
  • Peer Reviewed
• [Journal Article] Reconstruction of a seminiferous tubule-like structure in a 3 dimensional culture system of re-aggregated mouse neonatal testicular cells within a collagen matrix (2014)
  • Author(s): Zhang, J, Hatakeyama, J., Eto, K. and Abe, S.-I.
  • Journal Title: Gen Comp Endocrinol Volume: (in press) Pages: 121-132
  • DOI: 10.1016/j.ygcen.2014.03.030
  • Peer Reviewed / Open Access
• [Journal Article] Hes repressors are essential regulators of hematopoietic stem cell development downstream of Notch signaling (2013)
  • Author(s): Guiu J, Shimizu R, D'Altri T, Fraser ST, Hatakeyama J, Bresnick EH, Kageyama R, Dzierzak E, Yamamoto M, Espinosa L, and Bigas A
  • Journal Title: J Exp Med Volume: 210(1) Pages: 71-84
  • Peer Reviewed
• [Journal Article] Hes repressors are essential regulators of Hematopoietic Stem Cell Development downstream of Notch signaling. (2013)
  • Author(s): Guiu J, Shimizu R, D’Altri T, Fraser ST, Hatakeyama J, Bresnick EH , Kageyama R, Dzierzak E, Yamamoto M, Espinosa L and Bigas A.
  • Journal Title: Journal of Experimental Medicine Volume: 210
  • Peer Reviewed
• [Presentation] Pace control of neurogenesis regulated by transient retention of the apical endfoot of differentiating cells via Notch signaling (2014)
  • Author(s): Kenji Shimamura, Jun Hatakeyama, and Haruka Sato-Takemoto
  • Organizer: Cortical Development Conference 2014
  • Place of Presentation: Chania, Crete, Greece(ポスター発表)
• [Presentation] 「脳の形作り」の解明を目指して (2013)
  • Author(s): 畠山淳
  • Organizer: 動物・植物・生態学会三学会合同熊本例会
  • Place of Presentation: 熊本市(口頭発表)
  • Year and Date: 2013-11-16
  • Invited
• [Presentation] A novel boundary in the developing central nervous system (2013)
  • Author(s): Jun Hatakeyama, Andrew Lumsden, Ryoichiro Kageyama, and Kenji Shimamura
  • Organizer: Exciting Biologies
  • Place of Presentation: Savudrija, Croatia(ポスター発表)
• [Presentation] 「脳の形作り」の解明を目指して (2013)
  • Author(s): 畠山淳
  • Organizer: 熊本シンポジウム2014
  • Place of Presentation: 熊本市(口頭発表)
• [Presentation] The apical endfoot of nascent neuron controls the pace of neurogenesis through the regulation of notch signaling Neuro2013 (2013)
  • Author(s): Jun Hatakeyama, Yoshio Wakamatsu, Ryuichi Shigemoto, Akira Nagafuchi, and Kenji Shimamura
  • Organizer: 第36回日本神経科学会
  • Place of Presentation: 京都市(口頭発表)
• [Presentation] The apical endfoot of nascent neuron controls the pace of neurogenesis through the regulation of Notch signaling (2013)
  • Author(s): Jun Hatakeyama, Yoshio Wakamatsu, Ryuichi Shigemoto, Akira Nagafuchi, and Kenji Shimamura
  • Organizer: "The Making of a Vertebrate" CDB symposium 2013
  • Place of Presentation: Kobe, Japan(ポスター発表)
• [Presentation] The apical end foot of nascent neuron controls the pace of neurogenesis through the regulation of notch signaling. (2013)
  • Author(s): Hatakeyama J, Wakamatsu Y, Nagafuchi A, Shimamura K.
  • Organizer: Neuro2013 神経科学会
  • Place of Presentation: 国立京都国際会館（京都）
• [Presentation] 「脳の形作り」の解明を目指して (2013)
  • Author(s): 畠山 淳
  • Organizer: 動物植物生態学会三学会合同熊本例会
  • Place of Presentation: 熊本大学理学部（熊本）
  • Invited
• [Presentation] 脳の形作りの解明を目指して (2013)
  • Author(s): 畠山 淳
  • Organizer: 熊本シンポジウム２０１３
  • Place of Presentation: 熊本大学発生医学研究所 及び グリーンピア南阿蘇（熊本）
  • Invited
• [Presentation] A novel boundary in the developing central nervous system (2013)
  • Author(s): Hatakeyama J, Lumsden A, Kageyama R, Shimamura K.
  • Organizer: Exciting Biologies 2013
  • Place of Presentation: Kempinski Hotel (Savudrija、クロアチア)
• [Presentation] 生まれたてのニューロンのapical endfootによるNotchシグナルの調節を介した神経分化ペースのコントロール (2013)
  • Author(s): Jun Hatakeyama
  • Organizer: Neuro2013 第３６回日本神経科学学会
  • Place of Presentation: 京都国際会館
• [Presentation] The apical endfoot of nascent neuron controls the pace of neurogenesis through the regulation of Notch signaling. (2013)
  • Author(s): Jun Hatakeyama, Yoshio Wakamatsu, Ryuichi Shigemoto, Akira Nagafuchi, Kenji Shimamura
  • Organizer: CDB symposium 2013
  • Place of Presentation: 神戸理化学研究所CDB
• [Presentation] Afferent-dependent formation of the cytoarchitectonic features in mouse neocortical areas (2012)
  • Author(s): Haruka Sato-Takemoto, Jun Hatakeyama, Nobuhiko Yamamoto, and Kenji Shimamura
  • Organizer: The 35^<th> annual meeting of the Japan neuroscience society
  • Place of Presentation: Nagoya, Japan(ポスター発表)
• [Presentation] Tsukushi maintains the growth and undifferentiated properties of neuronal stem/ progenitor cells as a niche molecule (2012)
  • Author(s): Kunimasa Ohta, Yohei Shinmyo, Nahoko Kaneko, Yuki Hirata, Jun Hatakeyama, Masahiro Yamagucho, Kenji Shimamura, Kazunubu Sawamoto, Hideaki Tanaka, and Ayako Ito
  • Organizer: The 35^<th> annual meeting of the Japan neuroscience society
  • Place of Presentation: Nagoya, Japan(ポスター発表)
• [Presentation] Regulation of Notch signaling through the adherens junction in neurogenesis and somitogenesis. Joint Meeting of JSDB 45th & JSCB 64th (2012)
  • Author(s): Jun Hatakeyama, Yoshio Wakamatsu, Ryuichi Shigemoto, and Kenji Shimamura
  • Organizer: 日本発生生物学会、日本細胞生物学会合同年会
  • Place of Presentation: 兵庫県神戸市(口頭発表+ポスター発表)
• [Presentation] Regulation of Notch signaling through the adherens junction in neurogenesis and somitogenesis (2012)
  • Author(s): Jun Hatakeyama, Yoshio Wakamatsu, Ryuichi Shigemoto, Kenji Shimamura
  • Organizer: 第４５回日本発生生物学会
  • Place of Presentation: 神戸国際会議場