Molecular mechanism during development of bleomycin-induced pulmonary fibrosis model

• Project/Area Number: 24790374
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: The University of Tokyo
• Principal Investigator: SHIMAOKA TAKESHI 東京大学, 医学(系)研究科(研究院), 助教 (90422279)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 線維症 / 炎症

Research Abstract

We isolated CD45(-)CD31(-)Ter-119(-)GFP(-)PDGFR(+)ScaI(+)Mesenchymal stem cells (MSC) from Col-GFP Tg mice bone marrow. We established GFP-positive fibroblast differentiation from MSC by CTGF, bFGF and ascorbic acid. We pared down the candidates by transcriptome analysis and confirmed the involvement in fibroblast differentiation. We observed that several genes play an important role in fibroblast differentiation from MSC.

Research Products

• [Journal Article] Qualitative Rather than Quantitative Changes Are Hallmarks of Fibroblasts in Bleomycin-Induced Pulmonary Fibrosis (2013)
  • Author(s): Tatsuya Tsukui, Satoshi Ueha, Jun Abe, Shin-ichi Hashimoto, Shigeyuki Shichino, Takeshi Shimaoka, Francis H. W. Shand, Yasuka Arakawa, Kenshiro Oshima, Masahira Hattori, Yutaka
  • Journal Title: The American Journal of Pathology Volume: 3 Issue: 3 Pages: 758-773
  • DOI: 10.1016/j.ajpath.2013.06.005
  • Peer Reviewed
• [Journal Article] Distinct Roles for CXCR6+ and CXCR6- CD4+ T Cells in the Pathogenesis of Chronic Colitis (2013)
  • Author(s): Mandai Y, Takahashi D, Hase K, Obata Y, Furusawa Y, Ebisawa M, Nakagawa T, Sato T, Katsuno T, Saito Y, Shimaoka T, Yokosuka O, Yokote K, Ohno H
  • Journal Title: PloS One Volume: 8(6) Issue: 6 Pages: e65488-e65488
  • DOI: 10.1371/journal.pone.0065488
  • Peer Reviewed
• [Presentation] CXCL16 shows protective role in alpha-galactosylceramide suppression of pulmonary fibrosis development (2014)
  • Author(s): Shimaoka Takeshi, Arakawa Yasuka, Ueha Satoshi, Tsukui Tatsuya, Shichino Shigeyuki, Matsushima Kouji
  • Organizer: Keystone Symposia, Fibrosis : From bench to bedside
  • Place of Presentation: Colorado, USA
• [Presentation] CXCL16 shows protective role in alpha-galactosylceramide suppression of pulmonary fibrosis development (2014)
  • Author(s): Shimaoka Takeshi, Arakawa Yasuka, Ueha Satoshi, Tsukui Tatsuya, Shichino Shigeyuki, Matsushima Kouji
  • Organizer: Keystone Symposia, Fibrosis: From bench to bedside
  • Place of Presentation: Colorado, USA
• [Presentation] CXCL16 shows protective role in alpha-galactosylceramide suppression of pulmonary fibrosis development (2013)
  • Author(s): Shimaoka Takeshi, Arakawa Yasuka, Ueha Satoshi, Tsukui Tatsuya, Shichino Shigeyuki, Matsushima Kouji
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉