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Tissue stem cells transplantation activates the growth of hepatic progenitor cells in recipient rat livers.

Research Project

Project/Area Number 24790389
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Research InstitutionSapporo Medical University

Principal Investigator

ICHINOHE Norihisa  札幌医科大学, 医学部, 研究員 (80452978)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords細胞・組織 / 再生医学 / 肝幹・前駆細胞 / 生体組織工学 / 細胞移植 / 分化
Research Abstract

Oval cells and small hepatocytes (SHs) are known to be hepatic stem and progenitor cells, respectively. We have used Thy1 and CD44 as markers of stem and progenitor cells, respectively. In the present study we examined the differentiation capacity of Thy1+ cells and their contribution to liver regeneration. (1) Thy1+ cells could differentiate to the CD44+ SHs through Thy1+/CD44+ cells. Thy1+/CD44+ cells could also differentiate into bile duct cells in collagen sandwich culture. (2) Although transplanted Thy1+ cells could form only a small number of foci, , they could increase the number and the size of resident small hepatocyte-like progenitor cells (SHPCs). (3) The proliferation of SHs isolated was enhanced when the cells were indirectly co-cultured with Thy1+ cells. These results suggest that growth of hepatic progenitor cells may be enhanced by factors secreted by Thy1+ cells.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (41 results)

All 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (27 results) Book (4 results) Remarks (3 results)

  • [Journal Article] Sry HMG box protein 9-positive (Sox9+) epithelial cell adhesion molecule-negative (EpCAM-) biphenotypic cells derived from hepatocytes are involved in mouse liver regeneration.2014

    • Author(s)
      Tanimizu N, Nishikawa Y, Ichinohe N, Akiyama H, Mitaka T.
    • Journal Title

      J Biol Chem

      Volume: 289 Issue: 11 Pages: 7589-7598

    • DOI

      10.1074/jbc.m113.517243

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hepatic biliary epithelial cells acquire epithelial integrity but lose plasticity to differentiate into hepatocytes in vitro during development.2013

    • Author(s)
      Tanimizu N, Nakamura Y, Ichinohe N, Mizuguchi T, Hirata K, Mitaka T.
    • Journal Title

      J Cell Sci

      Volume: 126 Pages: 5239-5246

    • DOI

      10.1242/jcs.133082

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preoperative hepatocyte transplantation improves the survival of rats with non -alcoholic steatohepatitis-related cirrhosis after partial hepatectomy2013

    • Author(s)
      Nakamura Y, Mizuguchi T, Tanimizu N, Ichinohe N, Ooe H, Kawamoto M, Meguro M, Hirata K, Mitaka T.
    • Journal Title

      Cell Transplant

      Volume: e pub Pages: 1243-1254

    • DOI

      10.3727/096368913x668649

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differentiation capacity of hepatic stem/progenitor cells isolated from D-galactosamine-treated rat livers.2013

    • Author(s)
      Norihisa Ichinohe, Tanimizu N, Ooe H, Nakamura Y, Mizuguchi T, Hirata K, Kon J, Mitaka T
    • Journal Title

      Hepatology

      Volume: 57 Issue: 3 Pages: 1192-1202

    • DOI

      10.1002/hep.26084

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Proliferation of Rat Mesenchymal Stem Cells in Collagen Sponges Reinforced with Poly(Ethylene Terephthalate) Fibers by Stirring Culture Method2013

    • Author(s)
      Takamoto T, Ichinohe N, Tabata Y.
    • Journal Title

      Journal of biomaterials science. Polymer edition

      Volume: (In press) Issue: 13 Pages: 1741-1753

    • DOI

      10.1163/156856211x598184

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation of hepatic progenitor cells from the galactosamine-treated rat liver2012

    • Author(s)
      Ichinohe N, Kon J, Mitaka T
    • Journal Title

      Methods Mol Biol

      Volume: 826 Pages: 49-58

    • DOI

      10.1007/978-1-61779-468-1_5

    • ISBN
      9781617794674, 9781617794681
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Proliferation of rat small hepatocytes depends on follistatin expression2012

    • Author(s)
      Ooe H, Chen Q, Kon J, Sasaki K, Miyoshi H, Ichinohe N, Tanimizu N, Mitaka T
    • Journal Title

      J Cell Physiol

      Volume: 227 Issue: 6 Pages: 2363-2370

    • DOI

      10.1002/jcp.22971

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 細胞移植におけるレシピエント肝再生機構の解析2014

    • Author(s)
      市戸義久,谷水直樹,三高俊広
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都[口演発表]
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胆管反応を伴うマウス障害肝臓に出現する肝前駆細胞の増殖および分化能の解析2014

    • Author(s)
      谷水直樹,市戸義久,西川祐司,三高俊広
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都[口演発表]
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胆管反応を伴うマウス障害肝臓に出現する肝前駆細胞の増殖および分化能の解析2014

    • Author(s)
      谷水直樹
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都府京都市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞移植におけるレシピエント肝再生機構の解析2014

    • Author(s)
      市戸義久
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都府京都市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Retrorsine/部分肝切除モデルにおける肝再生機構の解析2013

    • Author(s)
      市戸義久,谷水直樹,三高俊広
    • Organizer
      第19回日本肝臓医生物研究会
    • Place of Presentation
      札幌
    • Year and Date
      2013-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Thy1陽性細胞移植における肝再生促進機序の解析2013

    • Author(s)
      市戸義久,谷水直樹,三高俊広
    • Organizer
      第46回北海道病理談話会
    • Place of Presentation
      札幌[口演発表]
    • Year and Date
      2013-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 障害肝より分離したThy1陽性細胞移植における肝再生促進機序の解析2013

    • Author(s)
      市戸義久,谷水直樹,三高俊広
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜[口演発表]
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Thy1-positive cells transplantation activates the growth of resident hepatic progenitor cells in rat livers2013

    • Author(s)
      Ichinohe N, Tanimizu N, Mitaka T
    • Organizer
      第20回肝細胞研究会
    • Place of Presentation
      大阪[口演発表]
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞移植におけるレシピエント肝再生機構の解析2013

    • Author(s)
      市戸義久,谷水直樹,今純子,三高俊広
    • Organizer
      第49回日本肝臓学会総会
    • Place of Presentation
      東京[口演発表]
    • Related Report
      2013 Final Research Report
  • [Presentation] 肝前駆細胞の分化誘導因子と成熟化能の解析2013

    • Author(s)
      市戸義久,谷水直樹,三高俊広
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      札幌[口演発表]
    • Related Report
      2013 Final Research Report
  • [Presentation] 肝前駆細胞の分化誘導因子と成熟化能の解析2013

    • Author(s)
      市戸義久
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      札幌市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞移植におけるレシピエント肝再生機構の解析2013

    • Author(s)
      三高俊広
    • Organizer
      第49回日本肝臓学会総会
    • Place of Presentation
      東京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Thy1-positive cells transplantation activates the growth of resident hepatic progenitor cells in rat livers.2013

    • Author(s)
      Ichinohe N,
    • Organizer
      第20回肝細胞研究会
    • Place of Presentation
      大阪府大阪市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Thy1陽性細胞移植における肝再生促進機序の解析2013

    • Author(s)
      市戸義久
    • Organizer
      第46回北海道病理談話会
    • Place of Presentation
      札幌市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Retrorsine/部分肝切除モデルにおける肝再生機構の解析2013

    • Author(s)
      市戸義久
    • Organizer
      第19回日本肝臓医生物研究会
    • Place of Presentation
      札幌市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 障害肝より分離したThy1陽性細胞移植における肝再生促進機序の解析2013

    • Author(s)
      市戸義久
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2012 Research-status Report
  • [Presentation] 細胞移植治療における肝幹?前駆細胞の有用性2012

    • Author(s)
      市戸義久,谷水直樹,三高俊広
    • Organizer
      第45回北海道病理談話会
    • Place of Presentation
      札幌[口演発表]
    • Year and Date
      2012-10-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Thy1陽性細胞移植における肝再生促進機序の解析2012

    • Author(s)
      市戸義久,中村幸雄,谷水直樹,三高俊広
    • Organizer
      第19回肝細胞研究会
    • Place of Presentation
      札幌[口演発表]
    • Year and Date
      2012-06-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Thy1陽性oval細胞における肝再生機構の解析2012

    • Author(s)
      市戸義久,今純子,大栄秀和,中村幸雄,谷水直樹,三高俊広
    • Organizer
      第48回日本肝臓学会総会
    • Place of Presentation
      金沢[口演発表]
    • Year and Date
      2012-06-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Thy1陽性細胞移植における肝再生機構の解析2012

    • Author(s)
      市戸義久,今純子,大栄秀和,中村幸雄,谷水直樹,三高俊広
    • Organizer
      第101回日本病理学会総会
    • Place of Presentation
      東京[口演発表]
    • Year and Date
      2012-04-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 肝幹細胞による再生医療における最新の知見2012

    • Author(s)
      水口徹,市戸義久,中村幸雄,柴田捻人,谷水直樹,吉川大和,三高俊広,平田公一
    • Organizer
      第42回日本創傷治癒学会
    • Place of Presentation
      札幌[シンポジウム]
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット肝硬変モデルにおける肝細胞移植の影響についての検討2012

    • Author(s)
      中村幸雄,水口徹,川本雅樹,目黒誠,市戸義久,谷水直樹,三高俊広
    • Organizer
      第19回肝細胞研究会
    • Place of Presentation
      札幌[ポスター発表]
    • Related Report
      2013 Final Research Report
  • [Presentation] Thy1陽性細胞移植における肝再生機構の解析2012

    • Author(s)
      市戸義久
    • Organizer
      第101回日本病理学会総会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Thy1陽性oval細胞における肝再生機構の解析2012

    • Author(s)
      市戸義久
    • Organizer
      第48回日本肝臓学会総会
    • Place of Presentation
      石川県金沢市
    • Related Report
      2012 Research-status Report
  • [Presentation] Thy1陽性細胞移植における肝再生促進機序の解析2012

    • Author(s)
      市戸義久
    • Organizer
      第19回肝細胞研究会
    • Place of Presentation
      北海道札幌市
    • Related Report
      2012 Research-status Report
  • [Presentation] ラット肝硬変モデルにおける肝細胞移植の影響についての検討2012

    • Author(s)
      中村幸雄
    • Organizer
      第19回肝細胞研究会
    • Place of Presentation
      北海道札幌市
    • Related Report
      2012 Research-status Report
  • [Presentation] 細胞移植治療における肝幹・前駆細胞の有用性2012

    • Author(s)
      市戸義久
    • Organizer
      第45回北海道病理談話会
    • Place of Presentation
      北海道札幌市
    • Related Report
      2012 Research-status Report
  • [Book] 各種肝病態における肝オーバル細胞・小型肝細胞の同定再生医療叢書5巻代謝系臓器2012

    • Author(s)
      市戸義久,大栄秀和,谷水直樹,三高俊.広
    • Related Report
      2013 Final Research Report
  • [Book] 肝再生における幹細胞の機能.肝胆膵、65巻、1号2012

    • Author(s)
      三高俊広,市戸義久,谷水直樹
    • Related Report
      2013 Final Research Report
  • [Book] 再生医療叢書5巻 代謝系臓器2012

    • Author(s)
      市戸義久
    • Total Pages
      8
    • Publisher
      朝倉書店
    • Related Report
      2012 Research-status Report
  • [Book] 肝胆膵2012

    • Author(s)
      三高俊広,
    • Total Pages
      9
    • Publisher
      アークメディア
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://web.sapmed.ac.jp/canpath/Tissue_Regeneration/Top_page.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 札幌医科大学医学部フロンティア医学研究所組織再生学部門

    • URL

      http://web.sapmed.ac.jp/canpath/Tissue_Regeneration/Tissue_Devel%26Regen.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] フロンティア医学研究所  組織再生学部門

    • URL

      http://web.sapmed.ac.jp/canpath/Tissue_Regeneration/Tissue_Devel%26Regen.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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