| Project/Area Number |
24790416
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 腸炎ビブリオ / ３型分泌装置 / エフェクター / 細胞死 / 細菌 / 3型分泌装置 / 細胞毒性 |
|---|
| Research Abstract |
Vibrio parahaemolyticus, a major food-borne pathogen, exhibits cytotoxicity that is dependent on its type III secretion system (T3SS1). Although a T3SS1 effector VepA plays a major role in the cytotoxicity, the mechanism was unknown.
In this study, a yeast genome-wide screen revealed that subunit c of V-ATPase is indispensable for the toxicity of VepA. Biochemical subcellular fractionation showed that VepA may be associated with lysosomes in infected cells. Although the toxicity of VepA was independent of the function of V-ATPases, VepA induces lysosomal membrane permeabilization and the leakage of contents from lysosomes. Therefore, these data suggest that the T3SS1 effector VepA targets subunit c of V-ATPase and induces the rupture of host cell lysosomes and subsequent cell death.
|
